Juvenile hormone controls early trypsin gene transcription in the midgut of Aedes aegypti

Early trypsin is a female‐specific protease present in the Aedes aegypti midgut during the first few hours after ingestion of a blood meal. The enzymatic activity of early trypsin plays an essential role in the transcriptional activation of the late trypsin gene, which encodes the major midgut endop...

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Bibliographic Details
Published in:Insect molecular biology 1997-02, Vol.6 (1), p.63-66
Main Authors: Noriega, F. G., Shah, D. K., Wells, M. A.
Format: Article
Language:English
Subjects:
Aedes - genetics
Aedes - metabolism
Animals
Digestive System
Female
Gene Expression Regulation
Genes, Insect
Juvenile Hormones - physiology
Methoprene - pharmacology
Transcription, Genetic
Trypsin - genetics
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Summary:Early trypsin is a female‐specific protease present in the Aedes aegypti midgut during the first few hours after ingestion of a blood meal. The enzymatic activity of early trypsin plays an essential role in the transcriptional activation of the late trypsin gene, which encodes the major midgut endoprotease involved in blood meal protein digestion. Transcription of the early trypsin gene is part of the normal post‐emergence maturation of the midgut in the adult female. Abdominal ligation within 1 h of emergence completely prevented the transcription of the early trypsin gene. Topically applied JH III or methoprene induced transcription of the early trypsin gene in ligated abdomens to levels similar to those observed in non‐ligated females. The induction of early trypsin transcription by JH is dose‐dependent and ‘head‐independent’, suggesting that factors coming from the neurosecretory axis are not required.
ISSN:0962-1075
1365-2583
DOI:10.1046/j.1365-2583.1997.00154.x