Peroxynitrite Induces the Conversion of Xanthine Dehydrogenase to Oxidase in Rabbit Liver

Effect of peroxynitrite (ONOO−) on the conversion of xanthine dehydrogenase to oxidase in rabbit liver was examined. ONOO−(25-200 μM) induced the conversion of xanthine dehydrogenase to oxidase in a dose-dependent manner. The addition of hydroxyl radical scavengers (mannitol and dimethyl sulfoxide)...

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Published in:Biochemical and biophysical research communications 1997-01, Vol.230 (2), p.476-479
Main Authors: Sakuma, Satoru, Fujimoto, Yohko, Sakamoto, Yoshihiko, Uchiyama, Takeshi, Yoshioka, Kumiko, Nishida, Hiroko, Fujita, Tadashi
Format: Article
Language:English
Subjects:
Animals
Cytosol - enzymology
Dimethyl Sulfoxide - pharmacology
Dithiothreitol - pharmacology
Dose-Response Relationship, Drug
Free Radical Scavengers - pharmacology
Hydroxyl Radical
Kinetics
Liver - enzymology
Male
Mannitol - pharmacology
Nitrates - pharmacology
Rabbits
Xanthine Dehydrogenase - metabolism
Xanthine Oxidase - metabolism
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Summary:Effect of peroxynitrite (ONOO−) on the conversion of xanthine dehydrogenase to oxidase in rabbit liver was examined. ONOO−(25-200 μM) induced the conversion of xanthine dehydrogenase to oxidase in a dose-dependent manner. The addition of hydroxyl radical scavengers (mannitol and dimethyl sulfoxide) gave no alteration in the ONOO−-induced conversion of xanthine dehydrogenase to oxidase, implying that the action of ONOO−is not due to hydroxyl radicals which may be formed from ONOO−. The experiment utilizing dithiothreitol also revealed that the action of ONOO−might be due to oxidation of sulfhydryl group of xanthine dehydrogenase. These results suggest that ONOO−has the potential to convert xanthine dehydrogenase to oxidase, and that this effect may be correlated with cytotoxic actions of ONOO−.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.5983