Binding of the N-terminal 63 kDa portion of connectin/titin to α-actinin as revealed by the yeast two-hybrid system

Connectin/titin is a 3000 kDa protein which links the myosin filament to the Z-line in vertebrate striated muscle sarcomeres. To search for the Z-line proteins to which connectin binds, the yeast two-hybrid system was applied using cDNA coding the N-terminal 63 kDa fragment of connectin. Two clones...

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Bibliographic Details
Published in:FEBS letters 1997-01, Vol.401 (1), p.65-67
Main Authors: Ohtsuka, Hiroshi, Yajima, Hirohiko, Maruyama, Koscak, Kimura, Sumiko
Format: Article
Language:English
Subjects:
Actinin - metabolism
Animals
Chickens
Connectin
DNA, Complementary
Enzyme-linked immunosorbent assay
Muscle Proteins - genetics
Muscle Proteins - metabolism
Muscle structure
Muscles - metabolism
Protein Binding
Protein Kinases - genetics
Protein Kinases - metabolism
Saccharomyces cerevisiae - genetics
Titin
Two-hybrid system
Z-line
α-Actinin
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Summary:Connectin/titin is a 3000 kDa protein which links the myosin filament to the Z-line in vertebrate striated muscle sarcomeres. To search for the Z-line proteins to which connectin binds, the yeast two-hybrid system was applied using cDNA coding the N-terminal 63 kDa fragment of connectin. Two clones coding the C-terminal half region of α-actinin (amino acids, 343–897 and 446–897) were obtained. Enzyme-linked immunosorbent assay clearly demonstrated the interactions of α-actinin and the N-terminal 63 kDa fragment of connectin in vitro. Thus it is concluded that the N-terminal 63 kDa portion of connectin binds to α-actinin in the Z-line of myofibrillar sarcomeres.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(96)01432-9