Crystallization and preliminary X-ray studies of plastocyanin from Silene expressed in E. coli

Plastocyanin from Silene (SilPc) expressed in E. coli has been crystallized in a form suitable for X‐ray diffraction analysis by a macroseeding method using ammonium sulfate as a precipitant in acetate buffer (pH = 5.5). These crystals belong to the trigonal space group P3121 or P3221 with lattice p...

Full description

Saved in:
Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 1997-01, Vol.53 (1), p.129-130
Main Authors: Li, C., Inoue, T., Gotowda, M., Hamada, K., Nishio, N., Hibino, T., Takabe, T., Kai, Y.
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plastocyanin from Silene (SilPc) expressed in E. coli has been crystallized in a form suitable for X‐ray diffraction analysis by a macroseeding method using ammonium sulfate as a precipitant in acetate buffer (pH = 5.5). These crystals belong to the trigonal space group P3121 or P3221 with lattice parameters a = b = 76.6, c = 65.5 Å, indicating an asymmetric unit containing two plastocyanin molecules. The crystals diffracted to at least 2.0 Å.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444996010724