Detection of human κ-opioid antibody using microresonators with integrated optical readout

Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accret...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2010-09, Vol.26 (1), p.195-201
Main Authors: Timurdogan, Erman, Ozber, Natali, Nargul, Sezin, Yavuz, Serhat, Kilic, M. Salih, Kavakli, I. Halil, Urey, Hakan, Alaca, B. Erdem
Format: Article
Language:English
Subjects:
Actuators
Antibodies
Antibodies - analysis
Antibodies - immunology
Bioelectricity
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensors
Biotechnology
Devices
Diffraction grating interferometry
Electromagnetic actuation
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
Human
Immunoassay - instrumentation
Integrated readout
Liquids
Methods. Procedures. Technologies
Micro-Electrical-Mechanical Systems - instrumentation
Microcantilever immunosensor
Miniaturization
Optical Devices
Protein–antibody interaction
Receptors
Receptors, Opioid, kappa - analysis
Receptors, Opioid, kappa - immunology
Refractometry - instrumentation
Resonator
Various methods and equipments
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Summary:Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical readout. A frequency resolution of 2 Hz is obtained. Together with a sensitivity of 7 ppm/(ng/ml) this leads to a minimum detectable antibody concentration of 5.7 ng/ml for a 50-kHz device. The measurement principle is shown to impart immunity to environmental noise, facilitate operation in liquid media and bring about the prospect for further miniaturization of actuator and readout leading to a portable biochemical sensor.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2010.06.004