Paracoccidioides brasiliensis antigen batches from the same isolate show immunological and biochemical differences

We investigated the occurrence of antigenic and biochemical variability among Paracoccidioides brasiliensis antigen batches prepared according to the same protocol. Initially (experiment #1), we analyzed two antigen lots of two human isolates (Bt1 & Bt2), cultured in two media (PYG: bactopeptone...

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Bibliographic Details
Published in:Mycopathologia (1975) 1996-01, Vol.135 (1), p.13-19
Main Authors: FRANCO, M, BAGAGLI, E, CUNHA, M, GASTAO CHAMMA, L, FECCHIO, D
Format: Article
Language:English
Subjects:
Antigens, Fungal - analysis
Antigens, Fungal - metabolism
Bacteriological Techniques
Biological and medical sciences
Culture Media - metabolism
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Fungal Proteins - analysis
Genetic Variation
Humans
Immunodiffusion
Immunoelectrophoresis, Two-Dimensional
Microbiology
Morphology, structure, chemical composition
Mycology
Paracoccidioides - immunology
Paracoccidioides - metabolism
Paracoccidioidomycosis - immunology
Paracoccidioidomycosis - microbiology
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Summary:We investigated the occurrence of antigenic and biochemical variability among Paracoccidioides brasiliensis antigen batches prepared according to the same protocol. Initially (experiment #1), we analyzed two antigen lots of two human isolates (Bt1 & Bt2), cultured in two media (PYG: bactopeptone, yeast extract, glucose; MMM: McVeigh & Morton medium) in SDS-PAGE and in two immunological tests (immunodiffusion-ID and footpad swelling test-FPT). Afterwards (experiment #2), we compared the antigenic profile of three antigen batches from three human isolates (Bt1, Bt2 & Bt3) by two-dimensional immunoelectrophoresis (2 D-IEP) against a reference system for P. brasiliensis antigens. In experiment #1, there were important intra- and inter-strain antigenic differences between batches of the fungal isolates cultured on both media. The block titration of the antigen batches for the immunological tests revealed correlation between protein concentration and biological activity in ID and no correlation in FPT. In experiment #2, the reference system for P. brasiliensis showed 26 antigen peaks. There were important differences between batches prepared from the same isolate and between batches from different isolates. Our data suggested the occurrence of instability in the synthesis of antigenic components by a same P. brasiliensis isolate, under controlled incubation conditions.
ISSN:0301-486X
1573-0832
DOI:10.1007/BF00436570