An eye color gene for the detection of transgenic non-drosophilid insects

A genetic marker for identifying transgenic Musca domestica by changes in eye color is described. The Drosophila melanogaster tryptophan oxygenase gene, vermilion (v), was tested for its ability to genetically complement the mutant tryptophan oxygenase gene in houseflies homozygous for green (ge). T...

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Bibliographic Details
Published in:	Insect biochemistry and molecular biology 1996-07, Vol.26 (7), p.641-644
Main Authors:	White, Lisa D., Coates, Craig J., Atkinson, Peter W., O'Brochta, David A.
Format:	Article
Language:	eng
Subjects:	Animals Animals, Genetically Modified color complementary DNA complementary genes compound eyes Drosophila melanogaster Drosophila melanogaster - enzymology Eye Color - genetics eye color mutants genetic complementation Genetic Markers genetic transformation Genetic Vectors Houseflies - embryology Houseflies - genetics Musca domestica mutants Phenotype promoter regions structural genes Transgenes transgenic animals transgenic markers tryptophan 2,3-dioxygenase Tryptophan Oxygenase - genetics
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Summary:	A genetic marker for identifying transgenic Musca domestica by changes in eye color is described. The Drosophila melanogaster tryptophan oxygenase gene, vermilion (v), was tested for its ability to genetically complement the mutant tryptophan oxygenase gene in houseflies homozygous for green (ge). The v cDNA, placed under the control of the hsp82 promoter of D. pseudoobscura was transiently expressed in M. domestica embryos homozygous for the tryptophan oxygenase gene, ge, resulting in the rescue of adult eye color. The use of a gene from D. melanogaster to complement an eye color mutant in Musca provides the opportunity to develop a gene vector system for M. domestica and a select group of other non-drosophilid insects in which homologous mutations exist.
ISSN:	0965-1748 1879-0240