RSC, an Essential, Abundant Chromatin-Remodeling Complex

A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex. At least three RSC subunits are related to SWI/SNF polypeptides: Sth1p, Rsc6p, and Rsc8p are significantly similar to...

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Bibliographic Details
Published in:Cell 1996-12, Vol.87 (7), p.1249-1260
Main Authors: Cairns, Bradley R., Lorch, Yahli, Li, Yang, Zhang, Mincheng, Lacomis, Lynne, Erdjument-Bromage, Hediye, Tempst, Paul, Du, Jian, Laurent, Brehon, Kornberg, Roger D.
Format: Article
Language:English
Subjects:
Acetyltransferases - metabolism
Adenosine Triphosphatases - chemistry
Adenosine Triphosphate - metabolism
Amino Acid Sequence
Cell Cycle Proteins
Chromatin - ultrastructure
Chromosomal Proteins, Non-Histone - physiology
DNA Helicases
DNA-Binding Proteins - physiology
Fungal Proteins - physiology
Gene Expression Regulation, Fungal
Genes, Fungal
Histone Acetyltransferases
Macromolecular Substances
Mass Spectrometry
Molecular Sequence Data
Nuclear Proteins - physiology
Nucleosomes - ultrastructure
Protein Structure, Secondary
RNA Polymerase II - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
Sequence Alignment
Sequence Homology, Amino Acid
Trans-Activators
Transcription Factors - chemistry
Transcription Factors - physiology
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Summary:A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex. At least three RSC subunits are related to SWI/SNF polypeptides: Sth1p, Rsc6p, and Rsc8p are significantly similar to Swi2/Snf2p, Swp73p, and Swi3p, respectively, and were identified by mass spectrometric and sequence analysis of peptide fragments. Like SWI/SNF, RSC exhibits a DNA-dependent ATPase activity stimulated by both free and nucleosomal DNA and a capacity to perturb nucleosome structure. RSC is, however, at least 10-fold more abundant than SWI/SNF complex and is essential for mitotic growth. Contrary to a report for SWI/SNF complex, no association of RSC (nor of SWI/SNF complex) with RNA polymerase II holoenzyme was detected.
ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(00)81820-6