ADP-ribosylation of serum proteins: evaluation as a potential tumor marker

Serum samples from cancer patients revealed elevated levels of in vitro ADP-ribosylation through non-enzymic binding of ADP-ribose to free acceptor sites on serum proteins. Low concentrations of serum ADP-ribose caused by high NAD glycohydrolase activity together with elevated rates of ADP-ribose tr...

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Bibliographic Details
Published in:Cancer letters 1996-11, Vol.108 (2), p.239-245
Main Authors: Albeniz, Işil Üstündaǧ, Nurten, Rüstem, Bermek, Engin
Format: Article
Language:English
Subjects:
Adenosine Diphosphate Ribose - metabolism
ADP-ribose transport
ADP-ribosylation
Adult
Aged
Biological and medical sciences
Biomarkers, Tumor - metabolism
Blood Proteins - metabolism
Carcinoembryonic Antigen - metabolism
CEA
Female
Host-tumor relations. Immunology. Biological markers
Humans
Male
Medical sciences
Middle Aged
NAD - metabolism
NAD glycohydrolase
Neoplasms - blood
Neoplasms - metabolism
Tumor marker
Tumors
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Summary:Serum samples from cancer patients revealed elevated levels of in vitro ADP-ribosylation through non-enzymic binding of ADP-ribose to free acceptor sites on serum proteins. Low concentrations of serum ADP-ribose caused by high NAD glycohydrolase activity together with elevated rates of ADP-ribose transport into erythrocytes appeared to account for undersaturation of the acceptor sites on serum proteins. ADP-ribosylation of serum proteins was assessed as an indicator of cancer disease, and an attempt was made to determine the correlation of ADP-ribosylation levels with carcinoembryonic antigen (CEA) values. Based on positive test results for all tumor patients and negative test results for all healthy controls, sensitivity and specificity of ADP-ribosylation as a tumor indicator were estimated as 67% and 95%, respectively. A close correlation appeared to exist with CEA ( r = 0.67; P < 0.001). Similarly, the changes in the levels of ADP-ribosylation correlated with the changes in the levels of CEA during the clinical course ( r = 0.58; P < 0.05).
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(96)04421-7