Molecular cloning and characterization of the mouse Kin17 gene coding for a Zn-finger protein that preferentially recognizes bent DNA

We report the isolation of the mouse Kin17 gene, located on chromosome 2, coding a nuclear Zn-finger protein that has a 39-residue region homologous to Escherichia coli RecA protein and that is recognized by anti-RecA antibodies. Kin17 protein preferentially binds to curved DNA in vitro and in vivo,...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1996-12, Vol.38 (2), p.238-242
Main Authors: TISSIER, A, KANNOUCHE, P, MAUFFREY, P, ALLEMAND, I, FRELAT, G, DEVORET, R, ANGULO, J. F
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cattle
Chickens
Cloning, Molecular
DNA
DNA-Binding Proteins - genetics
Dogs
Fundamental and applied biological sciences. Psychology
Genes. Genome
Humans
In Situ Hybridization
Introns
Macaca mulatta
Mice
Mice, Inbred BALB C
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nuclear Proteins
Rabbits
Rats
Rats, Sprague-Dawley
RNA-Binding Proteins
Zinc Fingers - genetics
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Summary:We report the isolation of the mouse Kin17 gene, located on chromosome 2, coding a nuclear Zn-finger protein that has a 39-residue region homologous to Escherichia coli RecA protein and that is recognized by anti-RecA antibodies. Kin17 protein preferentially binds to curved DNA in vitro and in vivo, suggesting a role in illegitimate recombination and in regulation of gene expression. We have shown that the Kin17 gene is about 8 kb in length and displays three exons and two introns. The 5' flanking region lacks a canonical TATAA box but presents several putative regulatory domains. A major transcription initiation site is located 322 nucleotides upstream of the translation start site. The 1.7-kb transcript of the Kin17 gene is weakly and ubiquitously expressed in murine tissues and cell lines as determined by Northern analysis. The cross-hybridization of Kin17 cDNA with the genomic DNA of other species in Southern analysis indicates the conservation of the gene among mammals and suggests that the Kin17 gene plays a conserved role in DNA metabolism.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1996.0623