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Cloning and characterization of hsc1+, a heat shock cognate gene of the fission yeast Schizosaccharomyces pombe
A heat shock cognate gene from the fission yeast Schizosaccharomyces pombe (Sp), designated hsc1+, was cloned. The putative translation product of hsc1+ contains 613 aa, with an estimated molecular mass of 67205 Da, and is more similar to the Saccharomyces cerevisiae (Sc) heat shock cognate protein...
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| Published in: | Gene 1996-11, Vol.181 (1/2), p.45-49 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 49 |
| container_issue | 1/2 |
| container_start_page | 45 |
| container_title | Gene |
| container_volume | 181 |
| creator | Oishi, K Sugiura, R Shuntoh, H Kuno, T |
| description | A heat shock cognate gene from the fission yeast Schizosaccharomyces pombe (Sp), designated hsc1+, was cloned. The putative translation product of hsc1+ contains 613 aa, with an estimated molecular mass of 67205 Da, and is more similar to the Saccharomyces cerevisiae (Sc) heat shock cognate protein SSB1 (69% identity) than the Sp heat-inducible ssp1+ gene product (41% identity). The hsc1+ mRNA was abundant during steady-state growth at 23 degrees C and decreased upon heat shock. Immunoblot analysis showed that the hsc1 protein is also abundant and constitutively expressed, however, we could not observe significant change in the protein level upon heat shock. DNA blot analyses indicated that hsc1+ is localized in Sp chromosome II, and suggested that the Sp genome contains a relatively smaller number of HSP70 genes compared with the Sc genome. |
| doi_str_mv | 10.1016/S0378-1119(96)00458-1 |
| format | article |
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The putative translation product of hsc1+ contains 613 aa, with an estimated molecular mass of 67205 Da, and is more similar to the Saccharomyces cerevisiae (Sc) heat shock cognate protein SSB1 (69% identity) than the Sp heat-inducible ssp1+ gene product (41% identity). The hsc1+ mRNA was abundant during steady-state growth at 23 degrees C and decreased upon heat shock. Immunoblot analysis showed that the hsc1 protein is also abundant and constitutively expressed, however, we could not observe significant change in the protein level upon heat shock. DNA blot analyses indicated that hsc1+ is localized in Sp chromosome II, and suggested that the Sp genome contains a relatively smaller number of HSP70 genes compared with the Sc genome.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(96)00458-1</identifier><identifier>PMID: 8973306</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Base Sequence ; Blotting, Northern ; Blotting, Western ; Cloning, Molecular ; DNA, Fungal - analysis ; Fungal Proteins - genetics ; genbank/d84218 ; gene expression ; genetic regulation ; heat shock cognate proteins ; heat shock proteins ; heat stress ; hsc1+ gene ; HSP70 Heat-Shock Proteins - genetics ; messenger RNA ; Molecular Sequence Data ; multigene family ; nucleotide sequences ; open reading frames ; restriction mapping ; Schizosaccharomyces - genetics ; Schizosaccharomyces pombe ; Schizosaccharomyces pombe Proteins ; Sequence Homology, Amino Acid ; structural genes</subject><ispartof>Gene, 1996-11, Vol.181 (1/2), p.45-49</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8973306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oishi, K</creatorcontrib><creatorcontrib>Sugiura, R</creatorcontrib><creatorcontrib>Shuntoh, H</creatorcontrib><creatorcontrib>Kuno, T</creatorcontrib><title>Cloning and characterization of hsc1+, a heat shock cognate gene of the fission yeast Schizosaccharomyces pombe</title><title>Gene</title><addtitle>Gene</addtitle><description>A heat shock cognate gene from the fission yeast Schizosaccharomyces pombe (Sp), designated hsc1+, was cloned. The putative translation product of hsc1+ contains 613 aa, with an estimated molecular mass of 67205 Da, and is more similar to the Saccharomyces cerevisiae (Sc) heat shock cognate protein SSB1 (69% identity) than the Sp heat-inducible ssp1+ gene product (41% identity). The hsc1+ mRNA was abundant during steady-state growth at 23 degrees C and decreased upon heat shock. Immunoblot analysis showed that the hsc1 protein is also abundant and constitutively expressed, however, we could not observe significant change in the protein level upon heat shock. DNA blot analyses indicated that hsc1+ is localized in Sp chromosome II, and suggested that the Sp genome contains a relatively smaller number of HSP70 genes compared with the Sc genome.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>Blotting, Western</subject><subject>Cloning, Molecular</subject><subject>DNA, Fungal - analysis</subject><subject>Fungal Proteins - genetics</subject><subject>genbank/d84218</subject><subject>gene expression</subject><subject>genetic regulation</subject><subject>heat shock cognate proteins</subject><subject>heat shock proteins</subject><subject>heat stress</subject><subject>hsc1+ gene</subject><subject>HSP70 Heat-Shock Proteins - genetics</subject><subject>messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>multigene family</subject><subject>nucleotide sequences</subject><subject>open reading frames</subject><subject>restriction mapping</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces pombe</subject><subject>Schizosaccharomyces pombe Proteins</subject><subject>Sequence Homology, Amino Acid</subject><subject>structural genes</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNo9kEtPwzAQhC0EKqXwEyp8QiAI-NHY8RFVvKRKHErP0cbZPCCJS-we2l9PqlbMZTWab-cwhEw5e-SMq6clkzqJOOfm1qg7xmbx4E7ImCfaRIzJ5JSM_5FzcuH9NxsUx2JERonRUjI1Jm7euK7uSgpdTm0FPdiAfb2DULuOuoJW3vL7Bwq0QgjUV87-UOvKDgLSEjvcM6FCWtTe71-2CD7Qpa3qnfNg95Wu3Vr0dO3aDC_JWQGNx6vjnZDV68vX_D1afL59zJ8XUSEkC5HNUORGyTxnItY8QavRisxolYlsBirmWQ5SG-AZK5QBJRXKArWwubQxE3JCbg696979btCHtK29xaaBDt3GpzpRQotZMoDTI7jJWszTdV-30G_T40JDfn3IC3AplH3t09VSMC4Zj4WSxsg_RolzbQ</recordid><startdate>19961128</startdate><enddate>19961128</enddate><creator>Oishi, K</creator><creator>Sugiura, R</creator><creator>Shuntoh, H</creator><creator>Kuno, T</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19961128</creationdate><title>Cloning and characterization of hsc1+, a heat shock cognate gene of the fission yeast Schizosaccharomyces pombe</title><author>Oishi, K ; Sugiura, R ; Shuntoh, H ; Kuno, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f230t-cbe2d963dd025718ec7ec2b976b2b4a651bda379a1b0f69a636e3fe72cd3c5023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>Blotting, Western</topic><topic>Cloning, Molecular</topic><topic>DNA, Fungal - analysis</topic><topic>Fungal Proteins - genetics</topic><topic>genbank/d84218</topic><topic>gene expression</topic><topic>genetic regulation</topic><topic>heat shock cognate proteins</topic><topic>heat shock proteins</topic><topic>heat stress</topic><topic>hsc1+ gene</topic><topic>HSP70 Heat-Shock Proteins - genetics</topic><topic>messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>multigene family</topic><topic>nucleotide sequences</topic><topic>open reading frames</topic><topic>restriction mapping</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces pombe</topic><topic>Schizosaccharomyces pombe Proteins</topic><topic>Sequence Homology, Amino Acid</topic><topic>structural genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oishi, K</creatorcontrib><creatorcontrib>Sugiura, R</creatorcontrib><creatorcontrib>Shuntoh, H</creatorcontrib><creatorcontrib>Kuno, T</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oishi, K</au><au>Sugiura, R</au><au>Shuntoh, H</au><au>Kuno, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of hsc1+, a heat shock cognate gene of the fission yeast Schizosaccharomyces pombe</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1996-11-28</date><risdate>1996</risdate><volume>181</volume><issue>1/2</issue><spage>45</spage><epage>49</epage><pages>45-49</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>A heat shock cognate gene from the fission yeast Schizosaccharomyces pombe (Sp), designated hsc1+, was cloned. The putative translation product of hsc1+ contains 613 aa, with an estimated molecular mass of 67205 Da, and is more similar to the Saccharomyces cerevisiae (Sc) heat shock cognate protein SSB1 (69% identity) than the Sp heat-inducible ssp1+ gene product (41% identity). The hsc1+ mRNA was abundant during steady-state growth at 23 degrees C and decreased upon heat shock. Immunoblot analysis showed that the hsc1 protein is also abundant and constitutively expressed, however, we could not observe significant change in the protein level upon heat shock. DNA blot analyses indicated that hsc1+ is localized in Sp chromosome II, and suggested that the Sp genome contains a relatively smaller number of HSP70 genes compared with the Sc genome.</abstract><cop>Netherlands</cop><pmid>8973306</pmid><doi>10.1016/S0378-1119(96)00458-1</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1996-11, Vol.181 (1/2), p.45-49 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Amino Acid Sequence amino acid sequences Base Sequence Blotting, Northern Blotting, Western Cloning, Molecular DNA, Fungal - analysis Fungal Proteins - genetics genbank/d84218 gene expression genetic regulation heat shock cognate proteins heat shock proteins heat stress hsc1+ gene HSP70 Heat-Shock Proteins - genetics messenger RNA Molecular Sequence Data multigene family nucleotide sequences open reading frames restriction mapping Schizosaccharomyces - genetics Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid structural genes |
| title | Cloning and characterization of hsc1+, a heat shock cognate gene of the fission yeast Schizosaccharomyces pombe |
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