Cloning and characterization of hsc1+, a heat shock cognate gene of the fission yeast Schizosaccharomyces pombe

A heat shock cognate gene from the fission yeast Schizosaccharomyces pombe (Sp), designated hsc1+, was cloned. The putative translation product of hsc1+ contains 613 aa, with an estimated molecular mass of 67205 Da, and is more similar to the Saccharomyces cerevisiae (Sc) heat shock cognate protein...

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Bibliographic Details
Published in:Gene 1996-11, Vol.181 (1/2), p.45-49
Main Authors: Oishi, K, Sugiura, R, Shuntoh, H, Kuno, T
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Base Sequence
Blotting, Northern
Blotting, Western
Cloning, Molecular
DNA, Fungal - analysis
Fungal Proteins - genetics
genbank/d84218
gene expression
genetic regulation
heat shock cognate proteins
heat shock proteins
heat stress
hsc1+ gene
HSP70 Heat-Shock Proteins - genetics
messenger RNA
Molecular Sequence Data
multigene family
nucleotide sequences
open reading frames
restriction mapping
Schizosaccharomyces - genetics
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins
Sequence Homology, Amino Acid
structural genes
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Summary:A heat shock cognate gene from the fission yeast Schizosaccharomyces pombe (Sp), designated hsc1+, was cloned. The putative translation product of hsc1+ contains 613 aa, with an estimated molecular mass of 67205 Da, and is more similar to the Saccharomyces cerevisiae (Sc) heat shock cognate protein SSB1 (69% identity) than the Sp heat-inducible ssp1+ gene product (41% identity). The hsc1+ mRNA was abundant during steady-state growth at 23 degrees C and decreased upon heat shock. Immunoblot analysis showed that the hsc1 protein is also abundant and constitutively expressed, however, we could not observe significant change in the protein level upon heat shock. DNA blot analyses indicated that hsc1+ is localized in Sp chromosome II, and suggested that the Sp genome contains a relatively smaller number of HSP70 genes compared with the Sc genome.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(96)00458-1