Detection of Cytomegalovirus DNA in Peripheral Blood of Patients Infected with Human Immunodeficiency Virus

Detection of cytomegalovirus (CMV) DNA can be facilitated by the polymerase chain reaction (PCR), an in vitro gene amplification technique. Twenty-eight CMV tissue culture isolates were examined by amplification of two separate CMV genes. All were found to contain CMV, although two of the isolates w...

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Bibliographic Details
Published in:The Journal of infectious diseases 1988-12, Vol.158 (6), p.1185-1192
Main Authors: Shibata, Darryl, Martin, W. John, Appleman, Maria D., Causey, Dennis M., Leedom, John M., Arnheim, Norman
Format: Article
Language:English
Subjects:
Acquired Immunodeficiency Syndrome - complications
AIDS
AIDS/HIV
Autoradiography
Biological and medical sciences
Blood
Cytomegalovirus
Cytomegalovirus - genetics
Cytomegalovirus - isolation & purification
Cytomegalovirus Infections - complications
Cytomegalovirus Infections - microbiology
Cytopathogenic Effect, Viral
DNA Probes
DNA, Viral - blood
DNA-Directed DNA Polymerase
Gene Amplification
HIV
human immunodeficiency virus
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Medical sciences
Oligomers
Original Articles
Plasmids
Polymerase chain reaction
Specimens
Templates, Genetic
Viruses
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Summary:Detection of cytomegalovirus (CMV) DNA can be facilitated by the polymerase chain reaction (PCR), an in vitro gene amplification technique. Twenty-eight CMV tissue culture isolates were examined by amplification of two separate CMV genes. All were found to contain CMV, although two of the isolates were positive for only one of the two genes. No detectable amplification occurred with human genomic or other viral DNA controls. The amplification products from as few as one CMV plaque-forming unit could be detected after the PCR. CMV DNA was detected in the blood of 14 of 27 patients with AIDS and one of six patients who were infected with human immunodeficiency virus but who did not have AIDS. Normal CMV-seropositive or -seronegative individuals did not have CMV DNA detected in their blood. The CMV PCR was more sensitive than the standard culture assay, can be completed in one to two days, uses only 20 µL of blood, and may be useful for rapidly detecting CMV in clinical specimens.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/158.6.1185