An improved method for the detection of Tospoviruses using the polymerase chain reaction

A reverse transcription-polymerase chain reaction (RT-PCR)-based assay for the detection of tomato spotted wilt virus (TSWV) has been improved and extended to enable the detection of additional Tospoviruses. In addition to TSWV-specific primers, two further pairs of primers have been designed, one p...

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Bibliographic Details
Published in:Journal of virological methods 1996-03, Vol.57 (1), p.109-115
Main Authors: Mumford, R.A., Barker, I., Wood, K.R.
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
DNA Primers
DNA, Viral - analysis
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
Groundnut ringspot virus
Impatiens necrotic spot virus
Microbiology
Molecular Sequence Data
Phytopathology. Animal pests. Plant and forest protection
Plant viral diagnostics
Plant viruses and viroids
Polymerase Chain Reaction - methods
RNA, Viral - analysis
RT-PCR
Techniques used in virology
Tomato chlorotic spot virus
Tomato spotted wilt virus
Tospovirus - genetics
Tospovirus - isolation & purification
Tospoviruses
Virology
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Summary:A reverse transcription-polymerase chain reaction (RT-PCR)-based assay for the detection of tomato spotted wilt virus (TSWV) has been improved and extended to enable the detection of additional Tospoviruses. In addition to TSWV-specific primers, two further pairs of primers have been designed, one pair which specifically detects impatiens necrotic spot virus (INSV) and another which detects all Tospoviruses tested, including TSWV, INSV, tomato chlorotic spot virus and groundnut ringspot virus. An improved, rapid RNA extraction method is also described.
ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(95)01975-8