A Role for Caveolin in Transport of Cholesterol from Endoplasmic Reticulum to Plasma Membrane

Caveolin is a 22-kDa membrane protein found associated with a coat material decorating the inner membrane surface of caveolae. A remarkable feature of this protein is its ability to migrate from caveolae directly to the endoplasmic reticulum (ER) when membrane cholesterol is oxidized. We now present...

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Bibliographic Details
Published in:The Journal of biological chemistry 1996-11, Vol.271 (46), p.29427-29435
Main Authors: Smart, E J, Ying, Y s, Donzell, W C, Anderson, R G
Format: Article
Language:English
Subjects:
Biological Transport
Caveolin 1
Caveolins
Cell Membrane - metabolism
Cells, Cultured
Cholesterol - metabolism
Endocytosis - drug effects
Endoplasmic Reticulum - metabolism
Humans
Membrane Proteins - metabolism
Progesterone - pharmacology
Tritium
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Summary:Caveolin is a 22-kDa membrane protein found associated with a coat material decorating the inner membrane surface of caveolae. A remarkable feature of this protein is its ability to migrate from caveolae directly to the endoplasmic reticulum (ER) when membrane cholesterol is oxidized. We now present evidence caveolin is involved in transporting newly synthesized cholesterol from the ER directly to caveolae. MA104 cells and normal human fibroblasts transported new cholesterol to caveolae with a half-time of ∼10 min. The cholesterol then rapidly flowed from caveolae to non-caveolae membrane. Cholesterol moved out of caveolae even when the supply of fresh cholesterol from the ER was interrupted. Treatment of cells with 10 μg/ml progesterone blocked cholesterol movement from ER to caveolae. Simultaneously, caveolin accumulated in the lumen of the ER, suggesting cholesterol transport is linked to caveolin movement. Caveolae fractions from cells expressing caveolin were enriched in cholesterol 3-4-fold, while the same fractions from cells lacking caveolin were not enriched. Cholesterol transport to the cell surface was nearly 4 times more rapid in cells expressing caveolin than in matched cells lacking caveolin.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.271.46.29427