Nested Retrotransposons in the Intergenic Regions of the Maize Genome

The relative organization of genes and repetitive DNAs in complex eukaryotic genomes is not well understood. Diagnostic sequencing indicated that a 280-kilobase region containing the maize Adh1-F and u22 genes is composed primarily of retrotransposons inserted within each other. Ten retroelement fam...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1996-11, Vol.274 (5288), p.765-768
Main Authors: SanMiguel, Phillip, Tikhonov, Alexander, Jin, Young-Kwan, Motchoulskaia, Natasha, Zakharov, Dmitrii, Melake-Berhan, Admasu, Springer, Patricia S., Edwards, Keith J., Lee, Michael, Avramova, Zoya, Bennetzen, Jeffrey L.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromosome mapping
Chromosomes, Artificial, Yeast
Classical genetics, quantitative genetics, hybrids
Corn
DNA, Plant - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Plant
Genetic aspects
Genetic loci
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Genome, Plant
Genomes
Genomics
Intergenic DNA
Milt
Molecular Sequence Data
Peat
Plant genetics
Pteridophyta, spermatophyta
Repetitive Sequences, Nucleic Acid
Retroelements
Retrotransposons
Transposons
Vegetals
Zea mays
Zea mays - genetics
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Summary:The relative organization of genes and repetitive DNAs in complex eukaryotic genomes is not well understood. Diagnostic sequencing indicated that a 280-kilobase region containing the maize Adh1-F and u22 genes is composed primarily of retrotransposons inserted within each other. Ten retroelement families were discovered, with reiteration frequencies ranging from 10 to 30,000 copies per haploid genome. These retrotransposons accounted for more than 60 percent of the Adh1-F region and at least 50 percent of the nuclear DNA of maize. These elements were largely intact and are dispersed throughout the gene-containing regions of the maize genome.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.274.5288.765