Dispersion and culture of magnocellular neurons from the supraoptic nucleus of the adult rat

A simple and efficient procedure for the dispersion and culture of magnocellular neurons of the adult rat hypothalamus was developed. The enzymatically and mechanically dispersed cells were highly viable and showed neurite outgrowth after 3 days of culture. The neurons could be maintained for more t...

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Bibliographic Details
Published in:Journal of neuroscience methods 1996, Vol.64 (1), p.13-18
Main Authors: Bilinski, Mario, Sánchez, Adelaida, Nicolini, Valeria González, Villar, Marcelo J., Tramezzani, Juan H.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Communication
Cell Survival
Cells, Cultured
Cytological Techniques
Fundamental and applied biological sciences. Psychology
General aspects. Models. Methods
Hypothalamus
Immunohistochemistry
Male
Monolayer culture
Neurons - cytology
Neurons - physiology
Oxytocin
Oxytocin - metabolism
Rats
Rats, Inbred Strains
Supraoptic Nucleus - cytology
Vasopressin
Vasopressins - metabolism
Vertebrates: nervous system and sense organs
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Summary:A simple and efficient procedure for the dispersion and culture of magnocellular neurons of the adult rat hypothalamus was developed. The enzymatically and mechanically dispersed cells were highly viable and showed neurite outgrowth after 3 days of culture. The neurons could be maintained for more than 4 weeks without any sign of deterioration. Light-optic immunocytochemistry of the cultured cells revealed the presence of many oxytocin- and vasopressin-immunoreactive neurons. The results indicate that the method may be suitable for many experimental purposes.
ISSN:0165-0270
1872-678X
DOI:10.1016/0165-0270(95)00030-5