Identification of a Plasmodium berghei antigen sharing common features with P. falciparum and P. chabaudi parasitophorous-vacuole membrane antigens

On the basis of immunological cross-reactivity, we identified a 43-kDa Plasmodium berghei antigen with homology to the exp-1 antigen from P. falciparium. The P. berghei antigen was recognized by an antibody directed against an epitope on the C-terminus of the P. falciparum exp-1 protein. This antige...

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Bibliographic Details
Published in:Parasitology research (1987) 1996, Vol.82 (2), p.130-135
Main Authors: TAN, T. M. C, KAY LIN GOH, LE NUGYEN BINH, TING, R. C. Y, KARA, U. A. K
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens, Protozoan - immunology
Antimalarials - pharmacology
Base Sequence
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
DNA, Protozoan - genetics
Fundamental and applied biological sciences. Psychology
Malaria, Falciparum - drug therapy
Malaria, Falciparum - immunology
Malaria, Falciparum - pathology
Mice
Molecular Sequence Data
Oligonucleotides - pharmacology
Peptides - chemistry
Plasmodium berghei - immunology
Plasmodium chabaudi - immunology
Plasmodium falciparum - drug effects
Plasmodium falciparum - immunology
Protozoa
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Summary:On the basis of immunological cross-reactivity, we identified a 43-kDa Plasmodium berghei antigen with homology to the exp-1 antigen from P. falciparium. The P. berghei antigen was recognized by an antibody directed against an epitope on the C-terminus of the P. falciparum exp-1 protein. This antigen is localized on the surface of the parasite and shares peptide sequence homology with the P. chabudi antigen Ag3008. To investigate further the role of the P. berghei antigen, we designed antisense phosphorothioate oligodeoxynucleotides (PS oligos) complementary to sequences of the exp-1 mRNA from P. falciparum. The PS oligos were capable of inhibiting the development of P. falciparum in vitro by 47%. In vivo, experiments in mice showed that the same PS oligos had the potential to extend the life span of mice infected with P. berghei by a factor of 2-4. The immunological cross-reactivity and the antisense inhibition of P. berghei parasite development in vivo indicate that this antigen may be a homologue of exp-1 from P. falciparum that has functional importance for parasite survival.
ISSN:0932-0113
1432-1955
DOI:10.1007/s004360050083