Oxygen-dependent regulation of energy metabolism in ascites tumor cells by nitric oxide

To understand the role of nitric oxide (NO) in the regulation of energy metabolism of tumor cells, its effect on the respiration and calcium homeostasis was examined with ascites hepatoma (AH130) cells under different oxygen tensions. NO reversibly inhibited the respiration and depolarized the membr...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1996-10, Vol.56 (19), p.4535-4540
Main Authors: NISHIKAWA, M, SATO, E. F, UTSUMI, K, INOUE, M
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Animals
Antimycin A - pharmacology
Ascites
Ascorbic Acid - pharmacology
Biological and medical sciences
Biological Transport - drug effects
Calcium - metabolism
Carcinoma, Hepatocellular - pathology
Electron Transport - drug effects
Energy Metabolism - physiology
Erythrocytes - physiology
General aspects (metabolism, cell proliferation, established cell line...)
Hemoglobins - metabolism
Liver Neoplasms - pathology
Male
Medical sciences
Membrane Potentials - drug effects
Mitochondria, Liver - metabolism
Neoplasm Transplantation
Nitric Oxide - physiology
Oxygen - physiology
Oxygen Consumption - drug effects
Rats
Rats, Inbred Strains
Rotenone - pharmacology
Succinates - pharmacology
Succinic Acid
Tetramethylphenylenediamine - pharmacology
Tumor cell
Tumor Cells, Cultured
Tumors
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Summary:To understand the role of nitric oxide (NO) in the regulation of energy metabolism of tumor cells, its effect on the respiration and calcium homeostasis was examined with ascites hepatoma (AH130) cells under different oxygen tensions. NO reversibly inhibited the respiration and depolarized the membrane potential of AH130 cells in an oxygen-dependent manner; the inhibition was more marked at physiologically low oxygen concentrations than at its high tensions. NO reversibly decreased the cellular ATP levels and elevated the cytosolic calcium, particularly under low oxygen concentrations. Since the peritoneal cavity is fairly anaerobic, the results suggested that small amounts of NO generated in this compartment might strongly affect the energy metabolism and calcium homeostasis of tumor cells in vivo.
ISSN:0008-5472
1538-7445