Membrane fluidity in Bacillus subtilis. Validity of homeoviscous adaptation

The validity of the principle of homeoviscous adaptation for Bacillus subtilis was tested by comparing fluorescence anisotropy (1,6-diphenyl-1,3,5-hexatriene) and electron-spin resonance (16-doxylstearate) measurements carried out in isolated plasma membranes and in phospholipid fractions. The physi...

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Bibliographic Details
Published in:Folia microbiologica 1988-01, Vol.33 (3), p.170-177
Main Authors: SVOBODOVA, J, JULAK, J, PILAR, J, SVOBODA, P
Format: Article
Language:English
Subjects:
Action of physical and chemical agents on bacteria
Adaptation, Physiological
Bacillus subtilis - analysis
Bacillus subtilis - physiology
Bacteriology
Biological and medical sciences
Electron Spin Resonance Spectroscopy
Fatty Acids - analysis
Fluorescence Polarization
Fundamental and applied biological sciences. Psychology
Membrane Fluidity
Microbiology
Phospholipids - analysis
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Summary:The validity of the principle of homeoviscous adaptation for Bacillus subtilis was tested by comparing fluorescence anisotropy (1,6-diphenyl-1,3,5-hexatriene) and electron-spin resonance (16-doxylstearate) measurements carried out in isolated plasma membranes and in phospholipid fractions. The physical measurements were supplemented by fatty-acid analysis. The results support our previous findings on intact cells. The thermoadaptive mechanism of B. subtilis manifested as an increase in relative proportion of branched anteiso-C15 and anteiso-C17 fatty acids, are not strong enough to compensate for the marked physical change of membrane fluidity induced by temperature decrease.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02925901