On-line urea kinetics in haemodiafiltration

Calculation of Kt/V and assessment of nutrition have so far been dependent upon off-line urea measurements of blood or dialysate samples. Here we describe a biosensor for on-line urea measurement during haemodiafiltration. Methods. The biosensor consisted of a cartridge containing covalently linked...

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Published in:Nephrology, dialysis, transplantation dialysis, transplantation, 1996-06, Vol.11 (6), p.1084-1092
Main Authors: Santoro, A, Tetta, C, Mandolfo, S, Arrigo, S, Berti, M, Colasanti, G, D'Amico, G, Imbasciati, E, Mazzocchi, C, Pacini, G, Spongano, M, Thomaseth, K, Wratten, M L, Zucchelli, P
Format: Article
Language:English
Subjects:
Adult
Aged
Automatic Data Processing
Biosensing Techniques
Equipment Design
Female
Humans
Male
Middle Aged
Models, Biological
Monitoring, Physiologic - instrumentation
Monitoring, Physiologic - methods
Renal Dialysis
Urea - pharmacokinetics
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Summary:Calculation of Kt/V and assessment of nutrition have so far been dependent upon off-line urea measurements of blood or dialysate samples. Here we describe a biosensor for on-line urea measurement during haemodiafiltration. Methods. The biosensor consisted of a cartridge containing covalently linked urease placed between two conductivity cells. The biosensor was placed on the outlet line of a haemofilter in series with a dialyser in order to obtain an aliquot of plasma ultrafiltrate for on-line measurement of urea. Urea nitrogen concentrations were highly correlated to the difference (Delta) in conductivity measured by the two conductivity cells both in aqueous solutions (in-vitro studies, y=-6. 676+32.12x, R2=0.998, P
ISSN:0931-0509
1460-2385
DOI:10.1093/ndt/11.6.1084