Protection against oral challenge three months after i.v. immunization of BALB/c mice with live Aro Salmonella typhimurium and Salmonella enteritidis vaccines is serotype (species)-dependent and only partially determined by the main LPS O antigen

The role of the main LPS O antigen in the specificity of protection as mediated by systemic mechanisms following immunization with live attenuated Aro Salmonella vaccines was studied in mice. Innately Salmonella-susceptible (Itys) BALB/c mice were immunized intravenously with a single dose of either...

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Bibliographic Details
Published in:Vaccine 1996-03, Vol.14 (4), p.251-259
Main Authors: Hormaeche, C E, Mastroeni, P, Harrison, J A, Demarco de Hormaeche, R, Svenson, S, Stocker, B A
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - blood
Antigens, Bacterial - immunology
Bacterial Vaccines - therapeutic use
Immunization
Lipopolysaccharides - immunology
Mice
Mice, Inbred BALB C
O Antigens
Polyisoprenyl Phosphate Sugars - immunology
Salmonella enteritidis
Salmonella enteritidis - immunology
Salmonella enteritidis - pathogenicity
Salmonella Infections, Animal - prevention & control
Salmonella typhimurium
Salmonella typhimurium - immunology
Salmonella Vaccines
Species Specificity
Typhoid-Paratyphoid Vaccines
Vaccines, Attenuated - therapeutic use
Virulence
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Summary:The role of the main LPS O antigen in the specificity of protection as mediated by systemic mechanisms following immunization with live attenuated Aro Salmonella vaccines was studied in mice. Innately Salmonella-susceptible (Itys) BALB/c mice were immunized intravenously with a single dose of either Salmonella typhimurium SL3261 aroA (LPS O4,5,12) or Salmonella enteritidis Se795aroA (LPS O1,9,12), and challenged orally 2-3 months later with either S. typhimurium C5 or S. enteritidis Thirsk. Nearly isogenic transductants of the two challenge strains expressing either their own LPS or that of the other serotype (S. typhimurium C5 O4 or O9, and S. enteritidis Thirsk O9 or O4) were also used. Both vaccines conferred similar high protection against the virulent strain of the homologous serotype expressing its own LPS. There was no protection against the heterologous serotype expressing its own LPS. However, when vaccinated mice were challenged with either the same serotype as the vaccine but expressing the heterologous LPS, or with the heterologous serotype expressing the LPS of the vaccine, protection was always lower than protection against the fully homologous serotype. Anti-smooth LPS antibodies showed higher titres against the homologous LPS, but with significant crossreactivity with the heterologous LPS. Antibodies to O-rough S. typhimurium and S. enteritidis LPS were present following immunization with either of the two vaccine strains. The LPS alone cannot fully account for the specificity of protection in this model; other (protein) antigens may be responsible. It remains to be seen whether there is a T-cell mediated component to the specificity of protection conferred by live Salmonella vaccines.
ISSN:0264-410X
1873-2518
DOI:10.1016/0264-410X(95)00249-Z