Magnesium deficiency and glucose metabolism in rat adipocytes

We examined the effect of reducing ambient and intracellular free Mg ion ([Mg]i) concentrations on insulin action in epididymal adipocytes from male Sprague-Dawley rats in terms of (1) cellular transport of nonmetabolizable 2-deoxyglucose, (2) [U- 14C]glucose oxidation to CO 2, and (3) d-[ 3H]glucos...

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Published in:Metabolism, clinical and experimental clinical and experimental, 1996-07, Vol.45 (7), p.838-843
Main Authors: Kandeel, Fouad R., Balon, Emilia, Scott, Stephen, Nadler, Jerry L.
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Adipose Tissue - drug effects
Adipose Tissue - metabolism
Animals
Biological Transport, Active
Carbon Dioxide - metabolism
Diabetes Complications
Diabetes Mellitus - metabolism
diet-related diseases
Glucose - metabolism
human nutrition
Humans
In Vitro Techniques
Insulin - pharmacology
Insulin Resistance - physiology
Magnesium - metabolism
Magnesium Deficiency - complications
Magnesium Deficiency - metabolism
Male
nutrition physiology
Oxidation-Reduction
Rats
Rats, Sprague-Dawley
Triglycerides - biosynthesis
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Summary:We examined the effect of reducing ambient and intracellular free Mg ion ([Mg]i) concentrations on insulin action in epididymal adipocytes from male Sprague-Dawley rats in terms of (1) cellular transport of nonmetabolizable 2-deoxyglucose, (2) [U- 14C]glucose oxidation to CO 2, and (3) d-[ 3H]glucose incorporation into triglycerides. There were no significant differences in basal or insulin-stimulated transport of 2-deoxyglucose between adipocytes cultured in physiologic (1.24 mmol) or low (0.16 mmol) Mg for up to 24 hours. In contrast, insulin-stimulated but not basal [U- 14C]glucose oxidation to CO 2 was significantly reduced in adipocytes cultured in low versus physiologic Mg ( P < .05 to .01). Similarly, there were no differences in basal glucose incorporation into triglycerides between cells cultured in low or physiologic Mg media for up to 24 hours. However, long-term (24-hour) but not short-term (2-hour) exposure of cells to low Mg was associated with a significant 30% reduction in insulin-stimulated d-[ 3H]glucose incorporation into triglycerides. When adipocytes incubated in low Mg were reincubated in high Mg (1.24 or 5 mmol) for 30 minutes, normal insulin-stimulated d-[ 3H]glucose incorporation into triglycerides was restored. Incubation of adipocytes in low Mg (0.16 mmol) for 24 hours resulted in a significant decrease in [Mg]i (264 ± 89 v 437 ± 125 μmol/cell [mean ± SEM]) as compared with cells incubated in physiologic Mg (1.24 mmol; P < .01). These data support a role for intracellular Mg deficiency in the development of insulin resistance and suggest that the effect occurs at a site(s) distal to glucose entry into the cell. The effect of Mg deficiency on insulin action appears to be reversible.
ISSN:0026-0495
1532-8600
DOI:10.1016/S0026-0495(96)90156-0