HIV-1 Reverse Transcriptase is a Target for Cytotoxic T Lymphocytes in Infected Individuals

Characterization of the host immune response to human immunodeficiency virus type 1 (HIV-1) is critical to the rational design of an effective AIDS vaccine. In this study, cytotoxic T lymphocytes (CTL) specific for HIV-1 reverse transcriptase (RNA-dependent DNA polymerase) were found in blood sample...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1988-04, Vol.240 (4848), p.64-66
Main Authors: Walker, Bruce D., Flexner, Charles, Paradis, Timothy J., Fuller, Thomas C., Hirsch, Martin S., Schooley, Robert T., Moss, Bernard
Format: Article
Language:English
Subjects:
Acquired immune deficiency syndrome
Acquired Immunodeficiency Syndrome - immunology
AIDS
AIDS/HIV
Antibodies
Antigens
Antigens, Viral - immunology
B lymphocytes
B-Lymphocytes - immunology
Biological and medical sciences
Cytotoxicity
DNA polymerases
DNA, Recombinant
Fovea
Ganglia
Genes, Viral
HIV
HIV (Viruses)
HIV - enzymology
HIV - genetics
HIV 1
HIV Seropositivity
HLA antigens
HLA Antigens - immunology
Human immunodeficiency virus 1
Humans
Immune response
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Immunotherapy
Medical research
Medical sciences
Retina
RNA-Directed DNA Polymerase - immunology
T cells
T lymphocytes
T-Lymphocytes, Cytotoxic - immunology
Vaccination & vaccines
Vaccinia virus - genetics
Vaccinia virus - immunology
Viral Vaccines - immunology
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Summary:Characterization of the host immune response to human immunodeficiency virus type 1 (HIV-1) is critical to the rational design of an effective AIDS vaccine. In this study, cytotoxic T lymphocytes (CTL) specific for HIV-1 reverse transcriptase (RNA-dependent DNA polymerase) were found in blood samples from HIV-1-infected individuals. CTL targets were prepared by immortalizing B cells from ten seropositive and six seronegative individuals, and then infecting these cells with recombinant vaccinia viruses containing HIV-1 genes. CTL directed against autologous B lymphoblasts expressing HIV-1 reverse transcriptase were detected in fresh blood samples from eight HIV-1 seropositive subjects, but in no seronegative controls. The effector cells were identified as major histocompatibility complex-restricted CD3+CD8+ lymphocytes. Because the HIV-1 pol gene is highly conserved among different isolates and generates both humoral and cellular immune responses, it bears consideration for inclusion in a candidate AIDS vaccine.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2451288