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Appearance of Birbeck Granule-like Structures in Anti-T6 Antibody-Treated Human Epidermal Langerhans Cells
We have previously demonstrated by immunogold labeling techniques that the T6 surface antigen of living human Langerhans cells (LC) of the skin is internalized by a process of receptor-mediated endocytosis (RME). Receptor-mediated endocytosis induced by the binding of the monoclonal anti-T6 antibody...
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Published in: | Journal of investigative dermatology 1988-03, Vol.90 (3), p.298-304 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | We have previously demonstrated by immunogold labeling techniques that the T6 surface antigen of living human Langerhans cells (LC) of the skin is internalized by a process of receptor-mediated endocytosis (RME). Receptor-mediated endocytosis induced by the binding of the monoclonal anti-T6 antibody BL6 involves coated pits, coated vesicles, endosomes, the smooth endoplasmic reticulum, and lysosomes. After RME, gold-labeled Birbeck granules (BGs) appear in the cell center. In the present study, by modifying the experimental conditions but retaining the presence of gold-labeled BL6, we have observed (in addition to the above-mentioned gold-labeled organelles) images that appear to correspond to BG-like structures. These structures, like the intracytoplasmic BGs, are pentalaminar and gold-labeled, but they differ from the intracytoplasmic BGs by their attachment to the cell membrane, their length, the frequent presence of a coat, and by prolongation by one or more coated pits. Birbeck granule like structures seem to result, at the ultrastructural level, from receptor ligand interactions and from modifications of the LC shape. |
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ISSN: | 0022-202X 1523-1747 |
DOI: | 10.1111/1523-1747.ep12456083 |