Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development

Summary Using a highly synchronous in vitro tuberization system, in combination with an amplified restriction fragment polymorphism (AFLP†)‐derived technique for RNA fingerprinting (cDNA‐AFLP‡), transcriptional changes at and around the time point of potato tuberization have been analyzed. The targe...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 1996-05, Vol.9 (5), p.745-753
Main Authors: Bachem, Christian W.B., Hoeven, Rutger S., Bruijn, Steef M., Vreugdenhil, Dick, Zabeau, Marc, Visser, Richard G.F.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Base Sequence
Biological and medical sciences
Carboxylic Ester Hydrolases
Cloning, Molecular
DNA Primers
DNA, Complementary
Economic plant physiology
Fructification, ripening. Postharvest physiology
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant
Genes, Plant
Genetic Techniques
Glucose-1-Phosphate Adenylyltransferase
Growth and development
Molecular Sequence Data
Nucleotidyltransferases - biosynthesis
Plant growth. Development of the storage organs
Plant physiology and development
Plant Proteins - biosynthesis
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
RNA, Messenger - biosynthesis
RNA, Plant - analysis
RNA, Plant - biosynthesis
Sequence Homology, Nucleic Acid
Solanum tuberosum
Solanum tuberosum - genetics
Solanum tuberosum - growth & development
Solanum tuberosum - physiology
Starch - biosynthesis
Time Factors
Transcription, Genetic
Vegetative apparatus, growth and morphogenesis. Senescence
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Summary:Summary Using a highly synchronous in vitro tuberization system, in combination with an amplified restriction fragment polymorphism (AFLP†)‐derived technique for RNA fingerprinting (cDNA‐AFLP‡), transcriptional changes at and around the time point of potato tuberization have been analyzed. The targeted expression analysis of a specific transcript coding for the major potato storage protein, patatin and a second transcript, coding for ADP‐glucose pyrophosphorylase, a key gene in the starch biosynthetic pathway is described. This paper confirms that kinetics of expression revealed by cDNA‐AFLP analysis are comparable to those found in Northern analysis. Furthermore, this paper reports the isolation and analysis of two tuberspecific transcript‐derived fragments (TDFs) coding for the lipoxygenase enzyme, which are differentially induced around the time point of tuber formation. Analysis of the two lox TDFs demonstrates that it is possible to dissect the expression modalities of individual transcripts, not independently detectable by Northern analysis. Finally, it is shown that using cDNA‐AFLP, rapid and simple verification of band identity may be achieved. The results indicate that cDNA‐AFLP is a broadly applicable technology for identifying developmentally regulated genes.
ISSN:0960-7412
1365-313X
DOI:10.1046/j.1365-313X.1996.9050745.x