Characterization of a rice sucrose-phosphate synthase-encoding gene

A rice genomic clone ( spsl) coding for sucrose phosphate synthase (SPS) was isolated and sequenced. Rice spsl contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter co...

Full description

Saved in:
Bibliographic Details
Published in:Gene 1996-05, Vol.170 (2), p.217-222
Main Authors: Valdez-Alarcón, Juan Jose, Ferrando, Marcelo, Salerno, Graciela, Jimenez-Moraila, Beatriz, Herrera-Estrella, Luis
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Base Sequence
Blotting, Southern
carbohydrate metabolism
carbon partitioning
Cloning, Molecular
DNA, Plant
enzyme activity
exons
genbank/u33175
Gene Expression
genetic regulation
Genome, Plant
Glucosyltransferases - genetics
hexosyltransferases
introns
leaves
messenger RNA
Molecular Sequence Data
nucleotide sequences
open reading frames
Oryza - enzymology
Oryza - genetics
Oryza sativa
plant development
Plant Leaves - growth & development
Plant Leaves - metabolism
promoter regions
Sequence Homology, Amino Acid
sink-source interaction
sps1 gene
structural genes
Sucrose synthesis
transcription (genetics)
transcription start point
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A rice genomic clone ( spsl) coding for sucrose phosphate synthase (SPS) was isolated and sequenced. Rice spsl contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter consensus sequences. Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome. Comparison of the rice, maize, potato and spinach SPS deduced amino acid (aa) sequences showed that these enzymes have a well conserved region comprising their first 700 aa, and a variable C-terminal region. Analysis of rice spsl expression showed that mRNA levels change during leaf development. SPS activity and mRNA were undetectable in roots.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00854-3