Effect of number of blood cultures and volume of blood on detection of bacteremia in children

OBJECTIVE: To determine whether bacteremia can be detected more rapidly and completely by (1) obtaining two blood cultures instead of one and/or (2) collecting a larger volume of blood. STUDY DESIGN: Prospective comparison of different strategies in 300 patients undergoing blood culture for suspecte...

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Bibliographic Details
Published in:The Journal of pediatrics 1996-02, Vol.128 (2), p.190-195
Main Authors: Isaacman, Daniel J., Karasic, Raymond B., Reynolds, Ellen A., Kost, Susanne I.
Format: Article
Language:English
Subjects:
Adolescent
Bacteremia - blood
Bacteremia - diagnosis
Bacteremia - microbiology
Bacteria - growth & development
Bacteria - isolation & purification
Bacterial diseases
Bacterial sepsis
Bacteriological Techniques
Biological and medical sciences
Blood - microbiology
Blood Specimen Collection
Child
Child, Preschool
Escherichia coli - growth & development
Escherichia coli - isolation & purification
Haemophilus influenzae - growth & development
Haemophilus influenzae - isolation & purification
Human bacterial diseases
Humans
Infant
Infectious diseases
Medical sciences
Phlebotomy
Prospective Studies
Salmonella - growth & development
Salmonella - isolation & purification
Streptococcus pneumoniae - growth & development
Streptococcus pneumoniae - isolation & purification
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Summary:OBJECTIVE: To determine whether bacteremia can be detected more rapidly and completely by (1) obtaining two blood cultures instead of one and/or (2) collecting a larger volume of blood. STUDY DESIGN: Prospective comparison of different strategies in 300 patients undergoing blood culture for suspected bacteremia. Each patient had two samples of blood, A (2 ml) and B (9.5 ml), obtained sequentially from separate sites. The B sample was divided into three aliquots: B1 (2 ml), B2 (6 ml), and ISO (1.5 ml, quantitative culture). RESULTS: A pathogen was isolated from one or more blood cultures in 30 patients (10% of cases). When measured at 24 hours, the pathogen recovery rate for the B2 sample (72%) was higher than that for the individual small-volume samples (A = 37%, B1 = 33%; p
ISSN:0022-3476
1097-6833
DOI:10.1016/S0022-3476(96)70388-8