Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and homology to other tospoviruses

The complete nucleotide sequence of the S RNA of peanut bud necrosis virus (PBNV) has been determined. The RNA is 3057 nucleotides in length, contains inverted repeats and two open reading frames (ORFs) with an ambisense coding strategy that are separated by an A + U-rich intergenic region. One ORF...

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Bibliographic Details
Published in:Archives of virology 1996, Vol.141 (1), p.85-98
Main Authors: Satyanarayana, T, Mitchell, S.E, Reddy, D.V.R, Brown, S, Kresovich, S, Jarret, R, Naidu, R.A, Demski, J.W
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Arachis hypogaea
Base Sequence
Biological and medical sciences
Capsid - chemistry
Capsid - genetics
Cloning, Molecular
DNA Primers
DNA, Complementary
Fundamental and applied biological sciences. Psychology
genbank/u27809
Genome, Viral
genomics
Molecular Sequence Data
Nicotiana tabacum
nucleotide sequences
Open Reading Frames
Phylogeny
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Polymerase Chain Reaction
progeny
RNA, Viral - chemistry
RNA, Viral - genetics
RNA, Viral - isolation & purification
Sequence Homology, Amino Acid
serological relationships
Species Specificity
Systematics. Structure, properties and multiplication. Genetics
Tospovirus
Tospovirus - classification
Tospovirus - genetics
Viral Core Proteins - chemistry
Viral Core Proteins - genetics
Viral Nonstructural Proteins - chemistry
Viral Nonstructural Proteins - genetics
viral proteins
watermelon silver mottle virus
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Summary:The complete nucleotide sequence of the S RNA of peanut bud necrosis virus (PBNV) has been determined. The RNA is 3057 nucleotides in length, contains inverted repeats and two open reading frames (ORFs) with an ambisense coding strategy that are separated by an A + U-rich intergenic region. One ORF (1320 nucleotides in the viral sense strand) encodes a Mr 49.5 kDa protein, identified as the nonstructural (NSs) protein based on similarity to published tospovirus sequences. The second ORF (831 nucleotides in virus complementary strand) encodes a Mr 30.6 kDa protein. This protein was identified as the nucleocapsid (N) protein based on sequence similarities. Amino acid sequence comparison of N and NSs proteins revealed identities of 22-34% with the reported tospovirus isolates of serogroups I, II, and III, whereas it had 82-86% identity with viruses in serogroup IV, watermelon silver mottle virus (WSMV) and tomato isolate of peanut bud necrosis virus (PBNV-To). Two subgenomic RNA species detected in PBNV infected tissue corresponded to the predicted sizes (1.65 and 1.4 kb) of the NSs and N mRNAs. The data presented show conclusively that PBNV should be included in serogroup IV, along with WSMV and PBNV-To.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01718590