Induction of axial mesoderm by zDVR-1, the zebrafish orthologue of Xenopus Vg1

The zebrafish DVR-1 (zDVR-1) gene, like Xenopus Vg1, is present maternally as an unprocessed precursor protein which is distributed ubiquitously along the future dorsoventral axis. Also, like Vg1, overexpression of zDVR-1 in zebrafish directs synthesis of more precursor, but no processed protein. Ho...

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Bibliographic Details
Published in:Developmental biology 1996-04, Vol.175 (1), p.108-117
Main Authors: Dohrmann, C E, Kessler, D S, Melton, D A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
DNA-Binding Proteins - biosynthesis
Embryonic Induction
Gene Expression Regulation, Developmental
Glycoproteins - metabolism
Goosecoid Protein
Homeodomain Proteins
Mesoderm - physiology
Molecular Sequence Data
Protein Precursors - metabolism
Protein Processing, Post-Translational
Repressor Proteins
Species Specificity
Tissue Distribution
Transcription Factors
Transforming Growth Factor beta - metabolism
Xenopus
Xenopus Proteins
Zebrafish - embryology
Zebrafish Proteins
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Description
Summary:The zebrafish DVR-1 (zDVR-1) gene, like Xenopus Vg1, is present maternally as an unprocessed precursor protein which is distributed ubiquitously along the future dorsoventral axis. Also, like Vg1, overexpression of zDVR-1 in zebrafish directs synthesis of more precursor, but no processed protein. However, the native zDVR-1 precursor is processed to mature protein when expressed in Xenopus. Like processed Vg1, mature zDVR-1 is a potent inducer of axial mesoderm. The parallels in expression pattern, apparent regulation of protein processing, and mesoderm-inducing activity support the hypothesis that localized protein processing controls production of a dorsal mesoderm inducer in these two species. Furthermore, using mutant mRNAs, we show that cleavage site sequences of the precursor protein are important in regulating protein processing.
ISSN:0012-1606
1095-564X
DOI:10.1006/dbio.1996.0099