Lipopolysaccharides of Helicobacter pylori Strains P466 and MO19:  Structures of the O Antigen and Core Oligosaccharide Regions

Lipopolysaccharides (LPS) from phenol−water extraction of dyspeptic (P466) and asymptomatic (MO19) strains of Helicobacter pylori were each isolated as water-soluble material of high relative molecular mass (high M r) and as water-insoluble gels of low M r. Chemical and spectroscopic analyses of the...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry (Easton) 1996-02, Vol.35 (7), p.2498-2504
Main Authors: Aspinall, Gerald O, Monteiro, Mario A
Format: Article
Language:English
Subjects:
Carbohydrate Conformation
Carbohydrate Sequence
Helicobacter pylori
Helicobacter pylori - chemistry
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens - chemistry
Oligosaccharides - chemistry
Spectrometry, Mass, Fast Atom Bombardment
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lipopolysaccharides (LPS) from phenol−water extraction of dyspeptic (P466) and asymptomatic (MO19) strains of Helicobacter pylori were each isolated as water-soluble material of high relative molecular mass (high M r) and as water-insoluble gels of low M r. Chemical and spectroscopic analyses of the soluble LPS and oligosaccharides liberated from the water-insoluble gels led to proposed structures for chains comprising the O antigen, intervening, and core regions. As in the LPS from the type strain NCTC 11637 [Aspinall, G. O., et al. (1996) Biochemistry 35, 000], the O antigen region of the P466 LPS is characterized by the presence of extended chains with fucosylated and nonfucosylated N-acetyllactosamine units, the former carrying α-l-fucopyranose units at O-3 of β-d-GlcNAc residues. This structure differs from that of the type strain in termination of the O chain by a Lewisy (Ley) antigenic determinant [α-l-Fuc(1→2)β-d-Gal(1→4)[α-l-Fuc(1→3)]β-d-GlcNAc] but also has internal Lewisx (Lex) units. The inner core region of the P466 LPS is indistinguishable from that in the type strain. In contrast, the O antigen region of the LPS from strain MO19 consists of a single Ley epitope linked via a 3-linked β-d-Gal to an intervening region on the basis of a sequence of 3-linked d-glycero-α-d-manno-heptose residues which is in turn linked to an inner core identical to that in the type strain and the P466 strain. Results in this and the preceding paper show that LPS from the three H. pylori strains display molecular mimicry of human cell surface glycoconjugates but may vary in the expression of Lex or Ley determinants, the degree of O antigen chain extension, or in the presence of an additional region between the inner core and the O antigen.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi951853k