The Saccharomyces cerevisiae OXA1 gene is required for the correct assembly of cytochrome c oxidase and oligomycin-sensitive ATP synthase

The nuclear gene OXA1 was first isolated in Saccharomyces cerevisiae and found to be required at a post-translational step in cytochrome c oxidase biogenesis, probably at the level of assembly. Mutations in OXA1 lead to a complete respiratory deficiency. The protein Oxa1p is conserved through evolut...

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Bibliographic Details
Published in:FEBS letters 1996-03, Vol.382 (1), p.111-115
Main Authors: Altamura, Nicola, Capitanio, Nazareno, Bonnefoy, Nathalie, Papa, Sergio, Dujardin, Geneviève
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - biosynthesis
Adenosine Triphosphatases - metabolism
ATP synthase
Carrier Proteins
Cytochrome c oxidase
Electron Transport Complex IV - biosynthesis
Electron Transport Complex IV - metabolism
Fungal Proteins - genetics
Fungal Proteins - physiology
Genes, Fungal - physiology
Heme - analogs & derivatives
Heme - analysis
Membrane Proteins - biosynthesis
Membrane Proteins - metabolism
Mitochondria - enzymology
Mitochondrial Proteins
Nuclear Proteins - genetics
Nuclear Proteins - physiology
Oligomycins
Open Reading Frames - genetics
OXA1
Respiratory complex assembly
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Sequence Deletion
Succinate Cytochrome c Oxidoreductase - metabolism
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Summary:The nuclear gene OXA1 was first isolated in Saccharomyces cerevisiae and found to be required at a post-translational step in cytochrome c oxidase biogenesis, probably at the level of assembly. Mutations in OXA1 lead to a complete respiratory deficiency. The protein Oxa1p is conserved through evolution and a human homolog has been isolated by functional complementation of a yeast oxa1 − mutant. In order to further our understanding of the role of Oxa1p, we have constructed two yeast strains in which the OXA1 open reading frame was almost totally deleted. Cytochrome spectra and enzymatic activity measurements show the absence of heme aa 3 and of a cytochrome c oxido-reductase activity and dramatic decrease of the oligomycin sensitive ATPase activity. Analysis of the respiratory complexes in non-denaturing gels reveals that Oxa1p is necessary for the correct assembly of the cytochrome c oxidase and the ATP synthase complex.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(96)00165-2