Structural Variation of the Pseudoautosomal Region between and within Inbred Mouse Strains

The pseudoautosomal region (PAR) is a segment of shared homology between the sex chromosomes. Here we report additional probes for this region of the mouse genome. Genetic and fluorescence in situ hybridization analyses indicate that one probe, PAR-4, hybridizes to the pseudoautosomal telomere and a...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1996-01, Vol.93 (1), p.171-175
Main Authors: Kipling, David, Wilson, Helen E., Thomson, Eric J., Lee, Muriel, Perry, Johanna, Palmer, Stephen, Ashworth, Alan, Cooke, Howard J.
Format: Article
Language:English
Subjects:
Alleles
Animals
Base Sequence
Chromosome Mapping
Chromosomes
Chromosomes, Artificial, Yeast
Cloning, Molecular
DNA
DNA Primers - chemistry
Genes
Genetic loci
Genetic variation
Genetics
Genomes
Human genetics
Inbred strains
Mice
Mice, Inbred Strains - genetics
Molecular Sequence Data
Polymorphism, Restriction Fragment Length
Rodents
Sex chromosomes
Telomere
Telomeres
X Chromosome
Y Chromosome
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Summary:The pseudoautosomal region (PAR) is a segment of shared homology between the sex chromosomes. Here we report additional probes for this region of the mouse genome. Genetic and fluorescence in situ hybridization analyses indicate that one probe, PAR-4, hybridizes to the pseudoautosomal telomere and a minor locus at the telomere of chromosome 9 and that a PCR assay based on the PAR-4 sequence amplifies only the pseudoautosomal locus (DXYHgu1). The region detected by PAR-4 is structurally unstable; it shows polymorphism both between mouse strains and between animals of the same inbred strain, which implies an unusually high mutation rate. Variation occurs in the region adjacent to a (TTAGGG)narray. Two pseudoautosomal probes can also hybridize to the distal telomeres of chromosomes 9 and 13, and all three telomeres contain DXYMov15. The similarity between these telomeres may reflect ancestral telomere-telomere exchange.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.1.171