Characterization of Human Adenovirus Proteinase Activity in Disrupted Virus Particles

Virus-coded proteinases are attractive targets for antiviral therapy; however, lack of quick, sensitive, quantitative, and selective assays for enzyme activity makes it difficult to characterize these proteinases and to screen large numbers of potential inhibitors. Here we describe new substrates fo...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 1996-03, Vol.217 (1), p.131-138
Main Authors: MCGRATH, WILLIAM J., ABOLA, A.PIA, TOLEDO, DIANA L., BROWN, MARK T., MANGEL, WALTER F.
Format: Article
Language:English
Subjects:
2,2'-Dipyridyl - analogs & derivatives
2,2'-Dipyridyl - pharmacology
adenovirus
Adenoviruses, Human - enzymology
Amino Acid Sequence
Cysteine Endopeptidases - drug effects
Cysteine Endopeptidases - metabolism
Detergents - pharmacology
Disulfides - pharmacology
HeLa Cells
Humans
Hydrogen-Ion Concentration
Molecular Sequence Data
Molecular Structure
Protease Inhibitors - pharmacology
Rhodamines
Solvents - pharmacology
Substrate Specificity
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Summary:Virus-coded proteinases are attractive targets for antiviral therapy; however, lack of quick, sensitive, quantitative, and selective assays for enzyme activity makes it difficult to characterize these proteinases and to screen large numbers of potential inhibitors. Here we describe new substrates for the adenovirus proteinase, fluorogenic Rhodamine-based substrates containing tetrapeptides corresponding to sequences cleaved in adenovirus precursor proteins. Proteinase activity in as few as 109disrupted virions could be quantitatively detected in a 30-min assay. With the substrate (Leu-Arg-Gly-Gly-NH)2–Rhodamine, theKmwas 1.4 μM,and theVmaxwas 3.24 pmol substrate hydrolyzed/sec/pmol virus. Enzyme activity was stimulated by dithiothreitol and inhibited by several serine-specific as well as cysteine-specific proteinase inhibitors. In a thiol protection experiment, the virion enzyme was shown to have a cysteine residue with an unusually low pKa, a pKasimilar to that of the active-site nucleophile of the cysteine proteinase papain. The curve ofVmaxas a function of pH is unlike the curve from papain and implied that there are at least three ionizable groups whose protonation state can affect catalysis—one with a pKaof 6.2, another with a pKaof 7.2, and a third with a pKaof 8.3.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1996.0100