Recombinant Pfs230, a Plasmodium falciparum gametocyte protein, induces antisera that reduce the infectivity of Plasmodium falciparum to mosquitoes

Six regions of malaria transmission-blocking target antigen, Pfs230, encoding 80% of the 363-kDa protein, were expressed as recombinant proteins in E. coli as fusions with maltose-binding protein (MBP). Antisera generated against amylose-purified recombinant Pfs230/MBP fusion proteins (r230/MBP.A–r2...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 1995-12, Vol.75 (1), p.33-42
Main Authors: Williamson, Kim C., Keister, David B., Muratova, Olga, Kaslow, David C.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Anopheles
Antibodies, Protozoan - biosynthesis
Antibody Formation
Antigen-Antibody Complex
Antigens, Protozoan - biosynthesis
Antigens, Protozoan - immunology
ATP-Binding Cassette Transporters
Carrier Proteins - biosynthesis
Cloning, Molecular
Culicidae
Culicidae - parasitology
Cysteine
Escherichia coli
Escherichia coli Proteins
Fluorescent Antibody Technique, Indirect
Malaria
Maltose-Binding Proteins
Mice
Mice, Inbred Strains
Molecular Sequence Data
Monosaccharide Transport Proteins
Pfs230
Plasmodium falciparum
Plasmodium falciparum - immunology
Plasmodium falciparum - pathogenicity
Polymerase Chain Reaction
Protozoan Proteins - biosynthesis
Protozoan Proteins - immunology
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - immunology
Sequence Homology, Amino Acid
Sexual-stage parasites
Transmission-blocking vaccine
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Summary:Six regions of malaria transmission-blocking target antigen, Pfs230, encoding 80% of the 363-kDa protein, were expressed as recombinant proteins in E. coli as fusions with maltose-binding protein (MBP). Antisera generated against amylose-purified recombinant Pfs230/MBP fusion proteins (r230/MBP.A–r230/MBP.F) all recognized the 360-kDa form of parasite-produced Pfs230 by immunoblot. However, only antisera against the four car☐y regions (C F) of Pfs230 and not the two amino regions (A and B) recognized the 310-kDa form of Pfs230, the form expressed on the surface of gametes. The data suggest that the 310-kDa form of Pfs230 arises from the cleavage of 50 kDa from the amino terminus of the 360-kDa form. Furthermore, antisera against r230/MBP.C bound to the surface of intact gametes and significantly reduced (by 71.2–89.8% (rank sum analysis, P < 0.01)) the infectivity of P. falciparum parasites to mosquitoes. This is the first report of a recombinant form of a P. falciparum gametocyte protein capable of inducing antisera that reduce malaria parasite infectivity to mosquitoes.
ISSN:0166-6851
1872-9428
DOI:10.1016/0166-6851(95)02507-3