Production of interferon γ messenger RNA by cells of non-immune origin

There is general agreement that IFN-γ is produced only by cells of immune origin (T-cells, NK cells, and recently macrophages). However, indirect evidence has suggested that undetectable, low levels of IFN-γ produced by cells of non-immune lineage, such as the murine line L-929, enhanced the antivir...

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Bibliographic Details
Published in:Cytokine (Philadelphia, Pa.) Pa.), 1995-11, Vol.7 (8), p.793-798
Main Authors: Rady, P.L., Cadet, P., Bui, T.K., Tyring, S.K., Baron, S., Stanton, G.J., Hughes, T.K.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - pharmacology
Carboxymethylcellulose Sodium - analogs & derivatives
Carboxymethylcellulose Sodium - pharmacology
Embryo, Mammalian
Fibroblasts
Fibroblasts - drug effects
Fibroblasts - immunology
Interferon Inducers - pharmacology
Interferon-alpha - immunology
Interferon-beta - immunology
interferon-gamma
Interferon-gamma - biosynthesis
Interferon-gamma - pharmacology
L Cells
messenger RNA
Mice
non-immune cells
Organ Specificity
Poly I-C - pharmacology
Polylysine - analogs & derivatives
Polylysine - pharmacology
Polymerase Chain Reaction
Recombinant Proteins
RNA, Messenger - biosynthesis
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Summary:There is general agreement that IFN-γ is produced only by cells of immune origin (T-cells, NK cells, and recently macrophages). However, indirect evidence has suggested that undetectable, low levels of IFN-γ produced by cells of non-immune lineage, such as the murine line L-929, enhanced the antiviral activity of IFNs-α and/or β following induction by agents such as the double stranded RNA poly ICLC. Since L-929 cells were one of the prototypic cell lines for studying murine IFN induction and action, we felt that it would be important to validate this observation by detection of the mRNA for IFN-γ. If confirmed, it might indicate a role for IFN-γ in non-immune cells. The present investigations revealed that mouse L-929 fibroblasts produce IFN-γ message following exposure to conventional IFN-α/β inducers such as poly ICLC or Newcastle disease virus. In addition, we found that IFN-γ itself will induce its own message. We further show that this is not a phenomenon isolated to transformed cells since we found that normal mouse embryo fibroblasts also produced the message, however in a constitutive fashion.
ISSN:1043-4666
1096-0023
DOI:10.1006/cyto.1995.0095