Production of monocyte chemoattractant protein-1 by bovine glomerular endothelial cells

Production of monocyte chemoattractant protein-1 by bovine glomerular endothelial cells. To explore the role of glomerular endothelial cells (GEN) in the pathogenesis of glomerulonephritis, the in vitro production of monocyte chemoattractant protein-1 (MCP-1) by bovine GEN was determined by chemotax...

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Bibliographic Details
Published in:Kidney international 1995-12, Vol.48 (6), p.1866-1874
Main Authors: Kakizaki, Yoshiki, Waga, Shinobu, Sugimoto, Kazuhiko, Tanaka, Hiroshi, Nukii, Kiyotaka, Takeya, Motohiro, Yoshimura, Teizo, Yokoyama, Masaru
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Northern
Blotting, Western
Cell Division
Cells, Cultured
Chemokine CCL2 - biosynthesis
Chemokine CCL2 - genetics
Cytokines - metabolism
Endothelium - metabolism
Endothelium - pathology
Glomerulonephritis
Glomerulonephritis - metabolism
Glomerulonephritis - pathology
Humans
Immunohistochemistry
Kidney Glomerulus - metabolism
Kidney Glomerulus - pathology
Medical sciences
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
RNA, Messenger - metabolism
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Summary:Production of monocyte chemoattractant protein-1 by bovine glomerular endothelial cells. To explore the role of glomerular endothelial cells (GEN) in the pathogenesis of glomerulonephritis, the in vitro production of monocyte chemoattractant protein-1 (MCP-1) by bovine GEN was determined by chemotaxis assay, Northern and Western blot analysis, and immunocytochemistry. Monocyte chemotactic activity of GEN-conditioned media was detectable by a chemotaxis assay using human peripheral blood monocytes. Exposure to human recombinant interleukin-1β (IL-1β) and phorbol myristate acetate (PMA) significantly increased the chemotactic activity of GEN-conditioned media. A checkerboard analysis showed that the response of monocytes to GEN-conditioned media was truly chemotactic. Immunoadsorption with a monoclonal antibody to human MCP-1 reduced the chemotactic activity of GEN-conditioned media by 85%. Northern blot analysis revealed that MCP-1 mRNA was constitutively expressed by GEN and that IL-1β and tumor necrosis factor-α (TNF-α) increased MCP-1 mRNA levels in a dose- and timedependent manner. Furthermore, PMA induced an increase in MCP-1 mRNA levels, whereas dibutyryl cyclic AMP and forskolin had minimal effects. Inhibition study using protein kinase inhibitors revealed that MCP-1 mRNA expression induced by IL-1β and TNF-α was suppressed by the tyrosine kinase inhibitor genistein, not by the protein kinase C inhibitors staurosporine or H-7, or the protein kinase A inhibitor H-89, suggesting an important role of tyrosine kinase in the cytokine-induced MCP-1 gene expression. Dexamethasone had a small inhibitory effect on constitutive MCP-1 mRNA expression, but no effect on the induction by TNF-α. By immunoperoxidase staining and Western blot analysis using an anti-MCP-1 monoclonal antibody, MCP-1 protein was detected in untreated GEN and increased by exposure to TNF-α. These results demonstrate the cytokine-induced production of MCP-1 by GEN at gene and protein levels as well as bioactivity, and suggest that GEN may participate in the development of glomerulonephritis through the production of MCP-1.
ISSN:0085-2538
1523-1755
DOI:10.1038/ki.1995.485