High efficiency induction of papillomas in vivo using recombinant cottontail rabbit papillomavirus DNA

Plasmids containing cottontail rabbit papillomavirus (CRPV) DNA can induce papillomas in vivo, but efficiency has been low. The aim of the present investigation was to explore some of the technical variables involved in inoculation of rabbits with recombinant CRPV DNA in attempts to improve both yie...

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Bibliographic Details
Published in:Journal of virological methods 1995-10, Vol.55 (2), p.233-244
Main Authors: Kreider, John W., Cladel, Nancy M., Patrick, Susan D., Welsh, Patricia A., Diangelo, Susan L., Bower, Jill M., Christensen, Neil D.
Format: Article
Language:English
Subjects:
Acetone - chemistry
Animals
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Cell Transformation, Neoplastic
Cell Transformation, Viral
Cottontail rabbit papillomavirus
Cottontail rabbit papillomavirus - genetics
Cottontail rabbit papillomavirus - physiology
Cutaneous scarification
DNA, Viral - metabolism
Epidermal hyperplasia
Experimental viral diseases and models
Female
Hyperplasia - chemically induced
Infectious diseases
Male
Medical sciences
Papilloma
Papilloma - virology
Plasmids
Rabbits
Skin Neoplasms - virology
Tumors
Turpentine
Viral diseases
Viruses
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Summary:Plasmids containing cottontail rabbit papillomavirus (CRPV) DNA can induce papillomas in vivo, but efficiency has been low. The aim of the present investigation was to explore some of the technical variables involved in inoculation of rabbits with recombinant CRPV DNA in attempts to improve both yield and consistency of papilloma induction. It was found that induction of epidermal hyperplasia, with either a mixture of turpentine and acetone or phorbol esters, produced a marked increase in papilloma yield. An additional powerful factor was the use of very vigorous, cutaneous scarification, sufficient to penetrate the papillary dermis and produce bleeding. When used in combination, papilloma yields were consistent and often reached 90–100% of inoculated sites. A number of other variables which did not consistently affect papilloma yield were tested. These included bleb and puncture injections, plasmid dose, vector type, occlusive dressings, lipofection reagent, carrier DNA, and different methods for plasmid DNA extraction and purification. It is concluded that the most important variables in improving papilloma yields were prior induction of epidermal hyperplasia and vigorous cutaneous scarification.
ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(95)00062-Y