Loading…
cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite
A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public datab...
Saved in:
| Published in: | Molecular and biochemical parasitology 1995-07, Vol.73 (1), p.75-90 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c388t-470e6fd3e9d2078fc4cd4a54464abd91a85832b6bb74715d3b4f4f2e3984e2453 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c388t-470e6fd3e9d2078fc4cd4a54464abd91a85832b6bb74715d3b4f4f2e3984e2453 |
| container_end_page | 90 |
| container_issue | 1 |
| container_start_page | 75 |
| container_title | Molecular and biochemical parasitology |
| container_volume | 73 |
| creator | El-Sayed, Najib M.A. Alarcon, Clara M. Beck, John C. Sheffield, Val C. Donelson, John E. |
| description | A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a
Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 cDNAs display significant similarities to genes in unrelated organisms encoding metabolic enzymes, signal transduction proteins, transcription factors, ribosomal proteins, histones, a proliferation-associated protein and thimet oligopeptidase, among others. 313 of the cDNAs are not related to any other sequences in the databases. These cDNA ESTs provide new avenues of research for exploring both the novel trypanosome-specific genes and the genome organization of this parasite, as well as a resource for identifying trypanosome homologs to genes expressed in other organisms. |
| doi_str_mv | 10.1016/0166-6851(95)00098-L |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77753810</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>016668519500098L</els_id><sourcerecordid>15675359</sourcerecordid><originalsourceid>FETCH-LOGICAL-c388t-470e6fd3e9d2078fc4cd4a54464abd91a85832b6bb74715d3b4f4f2e3984e2453</originalsourceid><addsrcrecordid>eNqFkUuPFCEUhYnRjD2j_0ATVsZZlEIVr9qYTMZn0tHNuCYU3OrGdBcllx7tfy9ld2apCwLkfvdcOIeQF5y94Yyrt3WpRhnJX_fymjHWm2b9iKy40W3Ti9Y8JqsH5Cm5RPxRIamVuiAXRmrdSbYixb__ekPh95wBEQJF-HmAyQMtboM0jfQuH2c3JUx7R4d88BBp3qYAGGFCoHNO9zEAneAXjRPGzbZgPZREyxboENMubY6LznKdXXYYCzwjT0a3Q3h-3q_I948f7m4_N-tvn77c3qwb3xlTGqEZqDF00IeWaTN64YNwUggl3BB67ow0XTuoYdBCcxm6QYxibKHrjYBWyO6KvDrp1lfWb2Gx-4gedjs3QTqg1VrLznD2X5BLVUnZV1CcQJ8TYobRzjnuXT5azuySil0st4vltpf2byp2XdtenvUPwx7CQ9M5hlp_d6pDdeM-Qrbo4xJDiBl8sSHFfw_4A4VZnYY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15675359</pqid></control><display><type>article</type><title>cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite</title><source>ScienceDirect Freedom Collection</source><creator>El-Sayed, Najib M.A. ; Alarcon, Clara M. ; Beck, John C. ; Sheffield, Val C. ; Donelson, John E.</creator><creatorcontrib>El-Sayed, Najib M.A. ; Alarcon, Clara M. ; Beck, John C. ; Sheffield, Val C. ; Donelson, John E.</creatorcontrib><description>A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a
Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 cDNAs display significant similarities to genes in unrelated organisms encoding metabolic enzymes, signal transduction proteins, transcription factors, ribosomal proteins, histones, a proliferation-associated protein and thimet oligopeptidase, among others. 313 of the cDNAs are not related to any other sequences in the databases. These cDNA ESTs provide new avenues of research for exploring both the novel trypanosome-specific genes and the genome organization of this parasite, as well as a resource for identifying trypanosome homologs to genes expressed in other organisms.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/0166-6851(95)00098-L</identifier><identifier>PMID: 8577350</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; cDNA ; Cloning, Molecular ; Databases, Factual ; DNA Primers - genetics ; DNA, Complementary - genetics ; DNA, Protozoan - genetics ; Expressed sequence tag ; Gene Expression ; Gene Library ; Genes, Protozoan ; GTP-Binding Proteins - genetics ; Humans ; Molecular Sequence Data ; Protozoan Proteins - genetics ; Repetitive Sequences, Nucleic Acid ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid ; Trypanosoma brucei rhodesiense ; Trypanosoma brucei rhodesiense - genetics</subject><ispartof>Molecular and biochemical parasitology, 1995-07, Vol.73 (1), p.75-90</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-470e6fd3e9d2078fc4cd4a54464abd91a85832b6bb74715d3b4f4f2e3984e2453</citedby><cites>FETCH-LOGICAL-c388t-470e6fd3e9d2078fc4cd4a54464abd91a85832b6bb74715d3b4f4f2e3984e2453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8577350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El-Sayed, Najib M.A.</creatorcontrib><creatorcontrib>Alarcon, Clara M.</creatorcontrib><creatorcontrib>Beck, John C.</creatorcontrib><creatorcontrib>Sheffield, Val C.</creatorcontrib><creatorcontrib>Donelson, John E.</creatorcontrib><title>cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a
Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 cDNAs display significant similarities to genes in unrelated organisms encoding metabolic enzymes, signal transduction proteins, transcription factors, ribosomal proteins, histones, a proliferation-associated protein and thimet oligopeptidase, among others. 313 of the cDNAs are not related to any other sequences in the databases. These cDNA ESTs provide new avenues of research for exploring both the novel trypanosome-specific genes and the genome organization of this parasite, as well as a resource for identifying trypanosome homologs to genes expressed in other organisms.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>cDNA</subject><subject>Cloning, Molecular</subject><subject>Databases, Factual</subject><subject>DNA Primers - genetics</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Protozoan - genetics</subject><subject>Expressed sequence tag</subject><subject>Gene Expression</subject><subject>Gene Library</subject><subject>Genes, Protozoan</subject><subject>GTP-Binding Proteins - genetics</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Protozoan Proteins - genetics</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Trypanosoma brucei rhodesiense</subject><subject>Trypanosoma brucei rhodesiense - genetics</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqFkUuPFCEUhYnRjD2j_0ATVsZZlEIVr9qYTMZn0tHNuCYU3OrGdBcllx7tfy9ld2apCwLkfvdcOIeQF5y94Yyrt3WpRhnJX_fymjHWm2b9iKy40W3Ti9Y8JqsH5Cm5RPxRIamVuiAXRmrdSbYixb__ekPh95wBEQJF-HmAyQMtboM0jfQuH2c3JUx7R4d88BBp3qYAGGFCoHNO9zEAneAXjRPGzbZgPZREyxboENMubY6LznKdXXYYCzwjT0a3Q3h-3q_I948f7m4_N-tvn77c3qwb3xlTGqEZqDF00IeWaTN64YNwUggl3BB67ow0XTuoYdBCcxm6QYxibKHrjYBWyO6KvDrp1lfWb2Gx-4gedjs3QTqg1VrLznD2X5BLVUnZV1CcQJ8TYobRzjnuXT5azuySil0st4vltpf2byp2XdtenvUPwx7CQ9M5hlp_d6pDdeM-Qrbo4xJDiBl8sSHFfw_4A4VZnYY</recordid><startdate>19950701</startdate><enddate>19950701</enddate><creator>El-Sayed, Najib M.A.</creator><creator>Alarcon, Clara M.</creator><creator>Beck, John C.</creator><creator>Sheffield, Val C.</creator><creator>Donelson, John E.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19950701</creationdate><title>cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite</title><author>El-Sayed, Najib M.A. ; Alarcon, Clara M. ; Beck, John C. ; Sheffield, Val C. ; Donelson, John E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-470e6fd3e9d2078fc4cd4a54464abd91a85832b6bb74715d3b4f4f2e3984e2453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>cDNA</topic><topic>Cloning, Molecular</topic><topic>Databases, Factual</topic><topic>DNA Primers - genetics</topic><topic>DNA, Complementary - genetics</topic><topic>DNA, Protozoan - genetics</topic><topic>Expressed sequence tag</topic><topic>Gene Expression</topic><topic>Gene Library</topic><topic>Genes, Protozoan</topic><topic>GTP-Binding Proteins - genetics</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Protozoan Proteins - genetics</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Trypanosoma brucei rhodesiense</topic><topic>Trypanosoma brucei rhodesiense - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El-Sayed, Najib M.A.</creatorcontrib><creatorcontrib>Alarcon, Clara M.</creatorcontrib><creatorcontrib>Beck, John C.</creatorcontrib><creatorcontrib>Sheffield, Val C.</creatorcontrib><creatorcontrib>Donelson, John E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El-Sayed, Najib M.A.</au><au>Alarcon, Clara M.</au><au>Beck, John C.</au><au>Sheffield, Val C.</au><au>Donelson, John E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>1995-07-01</date><risdate>1995</risdate><volume>73</volume><issue>1</issue><spage>75</spage><epage>90</epage><pages>75-90</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><notes>ObjectType-Article-2</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-1</notes><notes>content type line 23</notes><notes>ObjectType-Article-1</notes><notes>ObjectType-Feature-2</notes><abstract>A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a
Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 cDNAs display significant similarities to genes in unrelated organisms encoding metabolic enzymes, signal transduction proteins, transcription factors, ribosomal proteins, histones, a proliferation-associated protein and thimet oligopeptidase, among others. 313 of the cDNAs are not related to any other sequences in the databases. These cDNA ESTs provide new avenues of research for exploring both the novel trypanosome-specific genes and the genome organization of this parasite, as well as a resource for identifying trypanosome homologs to genes expressed in other organisms.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8577350</pmid><doi>10.1016/0166-6851(95)00098-L</doi><tpages>16</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-6851 |
| ispartof | Molecular and biochemical parasitology, 1995-07, Vol.73 (1), p.75-90 |
| issn | 0166-6851 1872-9428 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77753810 |
| source | ScienceDirect Freedom Collection |
| subjects | Amino Acid Sequence Animals Base Sequence cDNA Cloning, Molecular Databases, Factual DNA Primers - genetics DNA, Complementary - genetics DNA, Protozoan - genetics Expressed sequence tag Gene Expression Gene Library Genes, Protozoan GTP-Binding Proteins - genetics Humans Molecular Sequence Data Protozoan Proteins - genetics Repetitive Sequences, Nucleic Acid Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Trypanosoma brucei rhodesiense Trypanosoma brucei rhodesiense - genetics |
| title | cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T19%3A44%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=cDNA%20expressed%20sequence%20tags%20of%20Trypanosoma%20brucei%20rhodesiense%20provide%20new%20insights%20into%20the%20biology%20of%20the%20parasite&rft.jtitle=Molecular%20and%20biochemical%20parasitology&rft.au=El-Sayed,%20Najib%20M.A.&rft.date=1995-07-01&rft.volume=73&rft.issue=1&rft.spage=75&rft.epage=90&rft.pages=75-90&rft.issn=0166-6851&rft.eissn=1872-9428&rft_id=info:doi/10.1016/0166-6851(95)00098-L&rft_dat=%3Cproquest_cross%3E15675359%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c388t-470e6fd3e9d2078fc4cd4a54464abd91a85832b6bb74715d3b4f4f2e3984e2453%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=15675359&rft_id=info:pmid/8577350&rfr_iscdi=true |