cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite

A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public datab...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 1995-07, Vol.73 (1), p.75-90
Main Authors: El-Sayed, Najib M.A., Alarcon, Clara M., Beck, John C., Sheffield, Val C., Donelson, John E.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
cDNA
Cloning, Molecular
Databases, Factual
DNA Primers - genetics
DNA, Complementary - genetics
DNA, Protozoan - genetics
Expressed sequence tag
Gene Expression
Gene Library
Genes, Protozoan
GTP-Binding Proteins - genetics
Humans
Molecular Sequence Data
Protozoan Proteins - genetics
Repetitive Sequences, Nucleic Acid
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Trypanosoma brucei rhodesiense
Trypanosoma brucei rhodesiense - genetics
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Summary:A total of 518 expressed sequence tags (ESTs) have been generated from clones randomly selected from a cDNA library and a spliced leader sub-library of a Trypanosoma brucei rhodesiense bloodstream clone. 205 (39%) of the clones were identified based on matches to 113 unique genes in the public databases. Of these, 71 cDNAs display significant similarities to genes in unrelated organisms encoding metabolic enzymes, signal transduction proteins, transcription factors, ribosomal proteins, histones, a proliferation-associated protein and thimet oligopeptidase, among others. 313 of the cDNAs are not related to any other sequences in the databases. These cDNA ESTs provide new avenues of research for exploring both the novel trypanosome-specific genes and the genome organization of this parasite, as well as a resource for identifying trypanosome homologs to genes expressed in other organisms.
ISSN:0166-6851
1872-9428
DOI:10.1016/0166-6851(95)00098-L