Low resolution mapping around the flavivirus resistance locus (Flv) on mouse chromosome 5

Although the phenomenon of innate resistance to flaviviruses in mice was recognized many years ago, it was only recently that the genetic locus (Flv) controlling this resistance was mapped to mouse Chromosome (Chr) 5. Here we report the fine mapping of the Flv locus, using 12 microsatellite markers...

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Bibliographic Details
Published in:Mammalian genome 1995-07, Vol.6 (7), p.454-458
Main Authors: Urosevic, N, Mansfield, J P, Mackenzie, J S, Shellam, G R
Format: Article
Language:English
Subjects:
Animals
Chromosome Mapping
Chromosomes
flavivirus
Flavivirus Infections - genetics
Genetic Linkage
Immunity, Innate - genetics
Mammalia
Mice - genetics
Mice - immunology
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred Strains
Microsatellite Repeats - genetics
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Summary:Although the phenomenon of innate resistance to flaviviruses in mice was recognized many years ago, it was only recently that the genetic locus (Flv) controlling this resistance was mapped to mouse Chromosome (Chr) 5. Here we report the fine mapping of the Flv locus, using 12 microsatellite markers which have recently been developed for mouse Chr 5. The new markers were genotyped in 325 backcross mice of both (C3H/HeJ x C3H/RV)F1 x C3H/HeJ and (BALB/c x C3H/RV)F1 x BALB/c backgrounds, relative to Flv. The composite genetic map that has been constructed identifies three novel microsatellite loci, D5Mit68, D5Mit159, and D5Mit242, tightly linked to the Flv locus. One of those loci, D5Mit159, showed no recombinations with Flv in any of the backcross mice analyzed, indicating tight linkage (< 0.3 cM). The other two, D5Mit68 and D5Mit242, exhibited two and one recombinations with Flv (0.6 and 0.3 cM) respectively, defining the proximal and distal boundaries of a 0.9-cM segment around this locus. The proximal flanking marker, D5Mit68, maps to a segment on mouse Chr 5 homologous to human Chr 4. This, together with the previous data produced by our group, locates Flv to a region on mouse Chr 5 carrying segments that are conserved on either human Chr 4, 12, or 7, but present knowledge does not allow precise identification of the syntenic element.
ISSN:0938-8990
1432-1777
DOI:10.1007/BF00360653