Differential inhibition of reverse transcriptase and cellular DNA polymerase-α activities by lignans isolated from Chinese herbs, Phyllanthus myrtifolius Moon, and tannins from Lonicera japonica Thunb and Castanopsis hystrix

Two lignans, phyllamycin B and retrojusticidin B isolated from Phyllanthus myrtifolius Moon have been demonstrated to have a strong inhibitory effect on human immunodeficiency virus-1 reverse transcriptase activity (HIV-1 RT), but much less inhibitory effect on human DNA polymerase-α (HDNAP-α) activ...

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Bibliographic Details
Published in:Antiviral Research 1995-08, Vol.27 (4), p.367-374
Main Authors: Chang, Chia-Wen, Lin, Mei-Tsu, Lee, Shoei-Sheng, Liu, Karin C.S.Chen, Hsu, Feng-Lin, Lin, Jung-Yaw
Format: Article
Language:English
Subjects:
AIDS/HIV
Biological and medical sciences
Caffeoylquinate
DNA Polymerase II - antagonists & inhibitors
Drugs, Chinese Herbal - chemistry
Drugs, Chinese Herbal - pharmacology
Galloylquinate
Galloylshikimate
General pharmacology
HIV Reverse Transcriptase
human immunodeficiency virus 1
Humans
Kinetics
Lignan
Lignans - isolation & purification
Lignans - pharmacology
Lonicera japonica
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Reverse transcriptase inhibition
Reverse Transcriptase Inhibitors - isolation & purification
Reverse Transcriptase Inhibitors - pharmacology
RNA-Directed DNA Polymerase - drug effects
Structure-Activity Relationship
Tannins - isolation & purification
Tannins - pharmacology
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Summary:Two lignans, phyllamycin B and retrojusticidin B isolated from Phyllanthus myrtifolius Moon have been demonstrated to have a strong inhibitory effect on human immunodeficiency virus-1 reverse transcriptase activity (HIV-1 RT), but much less inhibitory effect on human DNA polymerase-α (HDNAP-α) activity. Fifty percent inhibitory concentrations of phyllamycin B and retrojusticidin B were determined to be 3.5 and 5.5 μM for HIV-1 RT, and 289 and 989 μM for HDNAP-α, respectively. The mode of inhibition was found to be non-competitive inhibition with respect to template-primer and triphosphate substrate. Several tannins such as caffeoylquinates (CQs) isolated from Lonicera japonica Thunb, galloylquinates (GQs) and galloylshikimates (GSs) purified from Castanopsis hystrix were shown to have a much less selective inhibitory effect on HIV-1 RT.
ISSN:0166-3542
1872-9096
DOI:10.1016/0166-3542(95)00020-M