Complete Sequence of the 43-kb Human Ribosomal DNA Repeat: Analysis of the Intergenic Spacer

We have sequenced the remaining 12.4 kb of the 30kb human ribosomal DNA intergenic spacer (IGS), which allows us to piece together both a complete IGS and a 43-kb rDNA unit. The sequence of the complete IGS reveals a collection of sequence motifs that can be correlated with functions known or expect...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1995-05, Vol.27 (2), p.320-328
Main Authors: Gonzalez, Iris L., Sylvester, James E.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
DNA - chemistry
DNA Mutational Analysis
DNA, Ribosomal - genetics
Fundamental and applied biological sciences. Psychology
Genes. Genome
Humans
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Repetitive Sequences, Nucleic Acid
Retroelements
Citations: Items that cite this one
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Summary:We have sequenced the remaining 12.4 kb of the 30kb human ribosomal DNA intergenic spacer (IGS), which allows us to piece together both a complete IGS and a 43-kb rDNA unit. The sequence of the complete IGS reveals a collection of sequence motifs that can be correlated with functions known or expected to reside in the rDNA repeat: modulation of transcription, recombination, initiation of DNA replication, and chromosomal organization. Finally, we find that IGS accumulates variation at a much higher rate than the transcribed regions. This finding leads us to correlate sequence character with types of mutation and sequence context with rate of mutation.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1995.1049