Regulation of T-cell receptor γ -chain RNA expression in murine Thy-1 + dendritic epidermal cells

The epidermis of normal mice contains two distinct populations of dendritic cells derived from the bone marrow, Ia+ Langerhans cells and Ia- cells that express the Thy-1 alloantigen. The Thy-1-bearing dendritic epidermal cells (Thy-1+ dEC) have a surface phenotype similar to that of very early T-lin...

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Bibliographic Details
Published in:Nature (London) 1987-07, Vol.328 (6127), p.263-266
Main Authors: Kuziel, William A, Takashima, Akira, Bonyhadi, Mark, Bergstresser, Paul R, Allison, James P, Tigelaar, Robert E, Tucker, Philip W
Format: Article
Language:English
Subjects:
Animals
Antigens, Differentiation, T-Lymphocyte
Antigens, Surface - analysis
Antigens, Surface - genetics
Biological and medical sciences
Concanavalin A - pharmacology
Dendritic Cells - physiology
Epidermis - cytology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gene Expression Regulation
Genes
Immunobiology
Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation
Mice
Peptide Fragments - genetics
Receptors, Antigen, T-Cell - genetics
Receptors, Antigen, T-Cell, gamma-delta
Recombination, Genetic
RNA, Messenger - genetics
Thy-1 Antigens
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Summary:The epidermis of normal mice contains two distinct populations of dendritic cells derived from the bone marrow, Ia+ Langerhans cells and Ia- cells that express the Thy-1 alloantigen. The Thy-1-bearing dendritic epidermal cells (Thy-1+ dEC) have a surface phenotype similar to that of very early T-lineage cells, produce IL-2-like growth factors and exhibit cytotoxicity which is not restricted by the major histocompatibility complex (MHC). The relationship of Thy-1+ dEC to the T-cell lineage is unclear. Most T lymphocytes bear a receptor for antigen composed of an alpha chain and a beta chain associated with a nonpolymorphic complex termed CD3 (T3). A minor population carries a receptor in which CD3 is associated with a gamma/delta complex. We have analysed clones of Thy-1+ dEC for rearrangement and expression of the genes for the alpha-, beta- and gamma-chains of the T-cell receptor (TCR). They do not express alpha or beta but do carry a gamma/delta complex. Activation of the cells with Con A is associated with a rapid decrease in the steady-state level of gamma-chain RNA. Because Thy-1+ dEC resemble early stage T lymphocytes, down-regulation of TCR expression may reflect a necessary event during T cell differentiation.
ISSN:0028-0836
1476-4687
DOI:10.1038/328263a0