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Catabolism of 2,4,6-trinitrotoluene by Mycobacterium vaccae
Mycobacterium vaccae strain JOB-5 cometabolized 2,4,6-trinitrotoluene (TNT) in the presence of propane as a carbon and energy source. Two novel oxidized metabolites, as well as several known reduced products, were generated during catabolism of TNT by M. vaccae. During the cometabolic process, there...
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| Published in: | Applied microbiology and biotechnology 1995-10, Vol.43 (5), p.937-945 |
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| Format: | Article |
| Language: | English |
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| container_end_page | 945 |
| container_issue | 5 |
| container_start_page | 937 |
| container_title | Applied microbiology and biotechnology |
| container_volume | 43 |
| creator | VANDERBERG, L. A PERRY, J. J UNKEFER, P. J |
| description | Mycobacterium vaccae strain JOB-5 cometabolized 2,4,6-trinitrotoluene (TNT) in the presence of propane as a carbon and energy source. Two novel oxidized metabolites, as well as several known reduced products, were generated during catabolism of TNT by M. vaccae. During the cometabolic process, there was transient production of a brown chromophore. This compound was identified as 4-amino-2,6-dinitrobenzoic acid. When M. vaccae was incubated with [14C]TNT and propane, 50% of the added radiolabel was incorporated into the cellular lipid fraction. These results suggest that ring cleavage occurred prior to the incorporation of radiolabelled carbon into phosphatidyl-L-serine, phosphatidylethanolamine, cardiolipin, and other polar lipids. |
| doi_str_mv | 10.1007/BF02431931 |
| format | article |
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A</creatorcontrib><creatorcontrib>PERRY, J. J</creatorcontrib><creatorcontrib>UNKEFER, P. J</creatorcontrib><title>Catabolism of 2,4,6-trinitrotoluene by Mycobacterium vaccae</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>Mycobacterium vaccae strain JOB-5 cometabolized 2,4,6-trinitrotoluene (TNT) in the presence of propane as a carbon and energy source. Two novel oxidized metabolites, as well as several known reduced products, were generated during catabolism of TNT by M. vaccae. During the cometabolic process, there was transient production of a brown chromophore. This compound was identified as 4-amino-2,6-dinitrobenzoic acid. When M. vaccae was incubated with [14C]TNT and propane, 50% of the added radiolabel was incorporated into the cellular lipid fraction. These results suggest that ring cleavage occurred prior to the incorporation of radiolabelled carbon into phosphatidyl-L-serine, phosphatidylethanolamine, cardiolipin, and other polar lipids.</description><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Biotransformation</subject><subject>Cardiolipins - analysis</subject><subject>Cell Fractionation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lipids - chemistry</subject><subject>Mission oriented research</subject><subject>Mycobacterium - metabolism</subject><subject>Mycobacterium vaccae</subject><subject>Oxidation-Reduction</subject><subject>Phosphatidylethanolamines - analysis</subject><subject>Phosphatidylserines - analysis</subject><subject>Physiology and metabolism</subject><subject>Propane - metabolism</subject><subject>Trinitrotoluene - metabolism</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqFkDtLxEAUhQdR1nW1sRdSiNVG77xnsdLFVWHFRutwZ3IjkTzWTCLk37tgsLU6xfdx4BzGzjlccwB7c78BoSRfSX7A5lxJkYLh6pDNgVudWr1yx-wkxk8ALpwxMzaz2hpt-JzdrrFH31ZlrJO2SMRSLU3ad2VT9l3bt9VADSV-TF7G0HoMPXXlUCffGALSKTsqsIp0NuWCvW8e3tZP6fb18Xl9t00_JIg-9VwVSE4Y6azUUulgyOdGB8m1d6iFckgCDBGhLhzIFQYFkIPwJuS5lQt29du769qvgWKf1WUMVFXYUDvEzO4XcgXyX5Fra4UB2IsXkzj4mvJs15U1dmM23bLnlxPHGLAqOmxCGf80aax0Qssfg2RuMw</recordid><startdate>19951001</startdate><enddate>19951001</enddate><creator>VANDERBERG, L. 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| ispartof | Applied microbiology and biotechnology, 1995-10, Vol.43 (5), p.937-945 |
| issn | 0175-7598 1432-0614 |
| language | eng |
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| source | SpringerLink Online Journals Archive Complete |
| subjects | Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Biotransformation Cardiolipins - analysis Cell Fractionation Fundamental and applied biological sciences. Psychology Lipids - chemistry Mission oriented research Mycobacterium - metabolism Mycobacterium vaccae Oxidation-Reduction Phosphatidylethanolamines - analysis Phosphatidylserines - analysis Physiology and metabolism Propane - metabolism Trinitrotoluene - metabolism |
| title | Catabolism of 2,4,6-trinitrotoluene by Mycobacterium vaccae |
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