Catabolism of 2,4,6-trinitrotoluene by Mycobacterium vaccae

Mycobacterium vaccae strain JOB-5 cometabolized 2,4,6-trinitrotoluene (TNT) in the presence of propane as a carbon and energy source. Two novel oxidized metabolites, as well as several known reduced products, were generated during catabolism of TNT by M. vaccae. During the cometabolic process, there...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1995-10, Vol.43 (5), p.937-945
Main Authors: VANDERBERG, L. A, PERRY, J. J, UNKEFER, P. J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Biotransformation
Cardiolipins - analysis
Cell Fractionation
Fundamental and applied biological sciences. Psychology
Lipids - chemistry
Mission oriented research
Mycobacterium - metabolism
Mycobacterium vaccae
Oxidation-Reduction
Phosphatidylethanolamines - analysis
Phosphatidylserines - analysis
Physiology and metabolism
Propane - metabolism
Trinitrotoluene - metabolism
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Summary:Mycobacterium vaccae strain JOB-5 cometabolized 2,4,6-trinitrotoluene (TNT) in the presence of propane as a carbon and energy source. Two novel oxidized metabolites, as well as several known reduced products, were generated during catabolism of TNT by M. vaccae. During the cometabolic process, there was transient production of a brown chromophore. This compound was identified as 4-amino-2,6-dinitrobenzoic acid. When M. vaccae was incubated with [14C]TNT and propane, 50% of the added radiolabel was incorporated into the cellular lipid fraction. These results suggest that ring cleavage occurred prior to the incorporation of radiolabelled carbon into phosphatidyl-L-serine, phosphatidylethanolamine, cardiolipin, and other polar lipids.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF02431931