Parvovirus B19 Clearance from Peripheral Blood after Acute Infection

The clearance of B19 parvovirus from peripheral blood was followed after acute infection to determine how long the virus is present in blood, even at low titer. The presence of B19 DNA in serum was investigated with dot blot hybridization during an epidemic. Fourteen patients positive for B19 DNA we...

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Bibliographic Details
Published in:The Journal of infectious diseases 1995-11, Vol.172 (5), p.1360-1363
Main Authors: Musiani, M., Zerbini, M., Gentilomi, G., Plazzi, M., Gallinella, G., Venturoli, S.
Format: Article
Language:English
Subjects:
Acute Disease
Antibodies, Viral - blood
Base Sequence
Biological and medical sciences
Blood
Blood - virology
Bone marrow
Concise Communications
DNA
DNA Primers
DNA, Viral - blood
Erythema Infectiosum - blood
Erythema Infectiosum - immunology
Erythema Infectiosum - virology
Female
Fever
Human parvovirus B19
Human viral diseases
Humans
Immunoglobulin G - blood
Immunoglobulin M - blood
Infections
Infectious diseases
Male
Medical sciences
Miscellaneous
Molecular Sequence Data
Parvovirus
parvovirus B19
Parvovirus B19, Human - genetics
Parvovirus B19, Human - isolation & purification
Polymerase chain reaction
Polymerase Chain Reaction - methods
Restriction Mapping
RNA Probes
Time Factors
Viral diseases
Virus Shedding
Viruses
Citations: Items that cite this one
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Summary:The clearance of B19 parvovirus from peripheral blood was followed after acute infection to determine how long the virus is present in blood, even at low titer. The presence of B19 DNA in serum was investigated with dot blot hybridization during an epidemic. Fourteen patients positive for B19 DNA were followed for up to 1 year for its presence in samples taken monthly, using both dot blot hybridization and nested polymerase chain reaction (PCR). All patients examined showed medium to high viremic titers (detectable with hybridization assay) only at the beginning of observation; later the virus titer decreased and was detectable only by nested PCR. Of the 14 patients followed, 13 were positive for B19 DNA by nested PCR for 2-6 months; 1 patient showed a persistent infection associated with chronic arthritis and was positive for B19 DNA for 1 year without clearance of the virus.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/172.5.1360