Dispersed pancreatic graft cryopreservation in the dog: In vivo assessment of preservation protocols

Dispersed canine pancreatic grafts were cryopreserved and the in vivo function was studied following intrasplenic autotransplantation. Four protocols were employed, examining the effects of cooling and thawing rates and cryoprotectant (dimethylsulfoxide) concentration on graft survival. The degree o...

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Bibliographic Details
Published in:Cryobiology 1987-06, Vol.24 (3), p.256-263
Main Authors: Walsh, Thomas N., Alderson, Derek, Farndon, John R.
Format: Article
Language:English
Subjects:
Animals
Cryoprotective Agents - pharmacology
Diabetes Mellitus, Experimental - therapy
Dimethyl Sulfoxide - pharmacology
Dogs
Evaluation Studies as Topic
Female
Freezing
Graft Survival
Male
Organ Preservation - methods
Pancreas Transplantation
Spleen
Transplantation, Autologous
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Summary:Dispersed canine pancreatic grafts were cryopreserved and the in vivo function was studied following intrasplenic autotransplantation. Four protocols were employed, examining the effects of cooling and thawing rates and cryoprotectant (dimethylsulfoxide) concentration on graft survival. The degree of graft injury by each protocol was assessed by examining the requirement for exogenous insulin following transplantation. Cooling at 5 °C/min and thawing at 80 °C/min allowed three successful grafts from seven when thawed at 80 °C/min using 1.4 or 2 M Me 2SO but only one success from eight when thawed at 8 °C/min. Of the seven experiments where successful preservation was achieved graft injury was estimated as less than 50% in four but for three it was probably greater than 50%. Each protocol exhibited considerable variability of islet survival. When sufficient islet mass was transplanted to restore fasting euglycaemia, graft function, as assessed by glucose-stimulated insulin release and intravenous glucose disposal, was identical to fresh grafts. Successful graft implantation, however, does not guarantee indefinite survival as six of seven grafts in this study became exhausted within 13 months of implantation.
ISSN:0011-2240
1090-2392
DOI:10.1016/0011-2240(87)90028-9