Mycobacterium tuberculosis strains unidentified using the IS6110 probe can be detected by oligonucleotides derived from the Mt308 sequence

Tuberculosis remains a problem worldwide. After having declined for 30 years, the overall incidence of tuberculosis has been on the rise since the mid-1980s. For example, in the United States, the number of new cases reported in 1992 represented a 20% increase over 1985. Moreover, the emergence of m...

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Published in:Research in microbiology 1995-05, Vol.146 (4), p.325-328
Main Authors: THIERRY, D, CHAVAROT, P, MARCHAL, G, LE THI, K. T, HO, M. L, NGUYEN, N. L, LE, N. V, LEDRU, S, FUMOUX, F, GUESDON, J.-L
Format: Article
Language:English
Subjects:
Adult
Aged
Bacteriology
Biological and medical sciences
Burkina Faso
DNA, Bacterial - analysis
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Microbiology
Middle Aged
Mycobacterium tuberculosis
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Oligonucleotide Probes
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Polymerase Chain Reaction - methods
Tuberculosis, Meningeal - diagnosis
Tuberculosis, Meningeal - microbiology
Tuberculosis, Pulmonary - diagnosis
Tuberculosis, Pulmonary - microbiology
Vietnam
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Summary:Tuberculosis remains a problem worldwide. After having declined for 30 years, the overall incidence of tuberculosis has been on the rise since the mid-1980s. For example, in the United States, the number of new cases reported in 1992 represented a 20% increase over 1985. Moreover, the emergence of multidrug-resistant tuberculosis (MDR-TB) has complicated the problem. Delayed diagnosis is considered to be one of the main factors contributing to several nosocomial outbreaks of MDR-TB. Indeed, patients with tuberculosis who remained infectious for prolonged periods transmitted the disease to other patients and health-care workers. According to the World Health Organisation, the number of people in Asia who develop tuberculosis because they are infected with both HIV and Mycobacterium tuberculosis is expected to multiply nearly sevenfold this decade. During the past 5 years, new diagnostic technologies based on DNA methods including PCR (polymerase chain reaction) and non-radioactive hybridization were developed to aid in tuberculosis control. The performance levels of these methods are directly dependent on the specificity of the target DNA sequence used to detect M. tuberculosis. Several DNA sequences with different specificities have been reported in the last few years and have been used to develop PCR tests for the direct identification of M. tuberculosis in non-cultured clinical specimens. Among them, an insertion sequence, IS6110, isolated from an M. tuberculosis cosmid library and found to be specific to mycobacteria belonging to the M. tuberculosis complex was used extensively in PCR tests (Brisson-Noeel et al., 1991; Thierry et al., 1990) by numerous laboratories. However, in Australia, Yuen et al., 1993, analysing by Southern blot hybridization 41 M. tuberculosis strains from patients of Vietnamese origin, found that four strains failed to hybridize with the IS6110 probe. Potentially, this would present problems in the application of PCR based on the use of IS6110 for tuberculosis diagnosis. We evaluated the frequency of such IS6110-negative strains among a series of strains isolated in Vietnam and Burkina-Faso, and we investigated the ability of the oligonucleotides derived from the Mt308 sequence already published (Thierry et al., 1992) to detect these particular strains.
ISSN:0923-2508
1769-7123
DOI:10.1016/0923-2508(96)81055-2