Isoforms of platelet-derived growth factor and its receptors in epiretinal membranes: Immunolocalization to retinal pigmented epithelial cells

Epiretinal membranes (ERMs) form on the inner surface of the retina in conjunction with various ocular disease processes, but the factors controlling their development are not understood. The predominant cell types involved are retinal pigmented epithelial (RPE) cells and retinal glia. Cultured RPE...

Full description

Saved in:
Bibliographic Details
Published in:Experimental eye research 1995-06, Vol.60 (6), p.607-619
Main Authors: Vinores, Stanley A., Henderer, Jeffrey D., Mahlow, Jeremy, Chiu, Charlie, Derevjanik, Nancy L., Larochelle, William, Csaky, Carl, Campochiaro, Peter A.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Eye Diseases - pathology
Humans
Immunohistochemistry
Medical sciences
Ophthalmology
Pigment Epithelium of Eye - chemistry
Platelet-Derived Growth Factor - analysis
Receptors, Platelet-Derived Growth Factor - analysis
Retina - pathology
Retinal Vessels - chemistry
Retinopathies
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Epiretinal membranes (ERMs) form on the inner surface of the retina in conjunction with various ocular disease processes, but the factors controlling their development are not understood. The predominant cell types involved are retinal pigmented epithelial (RPE) cells and retinal glia. Cultured RPE cells secrete platelet-derived growth factor (PDGF), which is chemotactic and mitogenic for both RPE cells and retinal glia and, therefore, could be involved in the development of ERMs. In the present study, we performed immunohistochemical staining for PDGF A chain (PDGF-A), PDGF B chain (PDGF-B), and both types of PDGF receptors (PDGF r α and PDGF r β ) on ERMs associated with various disease processes. PDGF-A is detected in most ERMs, regardless of the associated disease process, and it appears to be localized predominantly in RPE cells, recognized by the presence of pigment and the immunohistochemical demonstration of some or all of the following RPE-associated epitopes: class III β-tubulin, keratin, the 65-kDa microsomal protein recognized by the RPE9 antibody, and cellular retinaldehyde-binding protein. PDGF-B is found only in minor subpopulations of cells in about half of the ERMs evaluated and, with only occasional exceptions, appears to be localized almost entirely in blood-borne cells found in and around vessels in vascularized ERMs. Both PDGF r α and PDGF r β are demonstrated in most ERMs with neither isotype consistently predominating: they are found predominantly on RPE cells with many cells expressing both receptor types. ERMs with little or no RPE cell component contain little or no PDGF and PDGF receptor, whereas those in which the RPE cell represents the major cell type, have widespread PDGF and PDGF receptor positivity. These findings show that RPE cells in ERMs produce PDGF-A and PDGF α and PDGF β receptors and suggest that autocrine and paracrine stimulation with PDGF may be involved in ERM pathogenesis.
ISSN:0014-4835
1096-0007
DOI:10.1016/S0014-4835(05)80003-X